ArticlePloS one2011
Combining membrane potential imaging with L-glutamate or GABA photorelease.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Combining membrane potential and calcium imaging in brain slices using the voltage-sensitive dye ElectroFluor630 and the calcium indicator Calbryte520.Neurophotonics · 2026Article
- Kinetics and functional consequences of BK channels activation by N-type CaFrontiers in cellular neuroscience · 2024Article
- In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins.Nature communications · 2022Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- The Origin of Physiological Local mGluR1 Supralinear CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Two Distinct Sets of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Functional coupling of diverse voltage-gated Ca(2+) channels underlies high fidelity of fast dendritic Ca(2+) signals during burst firing.The Journal of physiology · 2016Article
- Branch specific and spike-order specific action potential invasion in basal, oblique, and apical dendrites of cortical pyramidal neurons.Neurophotonics · 2015Article
- Using simultaneous voltage and calcium imaging to study fast Ca(2+) channels.Neurophotonics · 2015Article
- Combining Membrane Potential Imaging with Other Optical Techniques.Advances in experimental medicine and biology · 2015Review
- Imaging GFP-based reporters in neurons with multiwavelength optogenetic control.Biophysical journal · 2014Article
- Imaging fast calcium currents beyond the limitations of electrode techniques.Biophysical journal · 2014Article
- All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.Nature methods · 2014Article
- Combined optogenetics and voltage sensitive dye imaging at single cell resolution.Frontiers in cellular neuroscience · 2014Article
- Economic and simple system to combine single-spot photolysis and whole-field fluorescence imaging.Journal of biomedical optics · 2013Article
- Rapid dopaminergic and GABAergic modulation of calcium and voltage transients in dendrites of prefrontal cortex pyramidal neurons.The Journal of physiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combining membrane potential imaging using voltage sensitive dyes with photolysis of L-glutamate or GABA allows the monitoring of electrical activity elicited by the neurotransmitter at different sub-cellular sites. Here we describe a simple system and some basic experimental protocols to achieve these measurements. We show how to apply the neurotransmitter and how to vary the dimension of the area of photolysis. We assess the localisation of photolysis and of the recorded membrane potential changes by depolarising the dendrites of cerebellar Purkinje neurons with L-glutamate photorelease using different experimental protocols. We further show in the apical dendrites of CA1 hippocampal pyramidal neurons how L-glutamate photorelease can be used to calibrate fluorescence changes from voltage sensitive dyes in terms of membrane potential changes (in mV) and how GABA photorelease can be used to investigate the phenomenon of shunting inhibition. We also show how GABA photorelease can be used to measure chloride-mediated changes of membrane potential under physiological conditions originating from different regions of a neuron, providing important information on the local intracellular chloride concentrations. The method and the proof of principle reported here open the gateway to a variety of important applications where the advantages of this approach are necessary.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.