ArticlePloS one2011
Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 58 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
58 citing papers in PubMed, 98 citations in OpenAlex.
- Boundary cap neural crest stem cells exhibit remarkable resilience to environmental stressors associated with International Space Station mission.NPJ microgravity · 2026Article
- Overexpression of Let-7a mitigates diploidization in mouse androgenetic haploid embryonic stem cells.iScience · 2024Article
- Selective miRNA quantitation with high-temperature thermal gel electrophoresis.Analytica chimica acta · 2023Article
- Differential Expression of ATM, NF-KB, PINK1 and Foxo3a in Radiation-Induced Basal Cell Carcinoma.International journal of molecular sciences · 2023Article
- Role of p53 in Regulating Radiation Responses.Life (Basel, Switzerland) · 2022Review
- miR-155, miR-21, and let-7a Expressions in MCF-10A and MCF-7 Cell Lines after Low to High Dose Irradiation.Cell journal · 2021Article
- Relationship between p53 status and the bioeffect of ionizing radiation.Oncology letters · 2021Review
- Mutations of p53 associated with pancreatic cancer and therapeutic implications.Annals of hepato-biliary-pancreatic surgery · 2021Review
- Epithelial-Mesenchymal Transition Gene Signature Related to Prognostic in Colon Adenocarcinoma.Journal of personalized medicine · 2021Article
- Survivin Expression Is Differentially Regulated by a Selective Cross-talk between RBM38 and miRNAs let-7b or miR-203a.Cancer research · 2021Article
- Clinical significance of microRNA-200 and let-7 families expression assessment in patients with ovarian cancer.Ecancermedicalscience · 2021Review
- A Perspective of Epigenetic Regulation in Radiotherapy.Frontiers in cell and developmental biology · 2021Review
- Article
- Repression of Irs2 by let-7 miRNAs is essential for homeostasis of the telencephalic neuroepithelium.The EMBO journal · 2020Article
- microRNAs Tune Oxidative Stress in Cancer Therapeutic Tolerance and Resistance.International journal of molecular sciences · 2019Review
- MicroRNA Networks Modulate Oxidative Stress in Cancer.International journal of molecular sciences · 2019Review
- Let-7 as biomarker, prognostic indicator, and therapy for precision medicine in cancer.Clinical and translational medicine · 2019Review
- Micromanaging aerobic respiration and glycolysis in cancer cells.Molecular metabolism · 2019Review
- Grape Seed Procyanidin B2 Protects Porcine Ovarian Granulosa Cells against Oxidative Stress-Induced Apoptosis by Upregulating let-7a Expression.Oxidative medicine and cellular longevity · 2019Article
- microRNA expression pattern as an ancillary prognostic signature for radiotherapy.Journal of translational medicine · 2018Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Genotoxic stressors, such as radiation, induce cellular damage that activates pre-programmed repair pathways, some of which involve microRNAs (miRNA) that alter gene expression. The let-7 family of miRNA regulates multiple cellular processes including cell division and DNA repair pathways. However, the role and mechanism underlying regulation of let-7 genes in response to stress have yet to be elucidated. In this study we demonstrate that let-7a and let-7b expression decreases significantly following exposure to agents that induce stress including ionizing radiation. This decrease in expression is dependent on p53 and ATM in vitro and is not observed in a p53(-/-) colon cancer cell line (HCT116) or ATM(-/-) human fibroblasts. Chromatin Immunoprecipitation (ChIP) analysis showed p53 binding to a region upstream of the let-7 gene following radiation exposure. Luciferase transient transfections demonstrated that this p53 binding site is necessary for radiation-induced decreases in let-7 expression. A radiation-induced decrease in let-7a and let-7b expression is also observed in radiation-sensitive tissues in vivo and correlates with altered expression of proteins in p53-regulated pro-apoptotic signaling pathways. In contrast, this decreased expression is not observed in p53 knock-out mice suggesting that p53 directly repress let-7 expression. Exogenous expression of let-7a and let-7b increased radiation-induced cytotoxicity in HCT116 p53(+/+) cells but not HCT116 p53(-/-) cells. These results are the first demonstration of a mechanistic connection between the radiation-induced stress response and the regulation of miRNA and radiation-induced cytotoxicity and suggest that this process may be a molecular target for anticancer agents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.