ArticleScience (New York, N.Y.)2011
Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding.
Article in Science (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
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Who cites it
129 citing papers in PubMed.
- Lipid-trap mass spectrometry identifies lipid-protein interactions in cells.Nature cell biology · 2026Article
- Quantitative Mapping of the Lipid Nanoenvironment around Transmembrane Proteins in Living Cells.ACS nano · 2026Article
- NaNature structural & molecular biology · 2025Article
- Article
- Mechanism of proton-powered c-ring rotation in a mitochondrial ATP synthase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions.eLife · 2024Article
- Bile acid fitness determinants of amBio · 2024Article
- Potential Antioxidant and Anti-Inflammatory Properties of Polyphenolic Compounds fromInternational journal of molecular sciences · 2024Article
- Deciphering Solution and Gas-Phase Interactions between Peptides and Lipids by Native Mass Spectrometry.Analytical chemistry · 2023Article
- Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions.bioRxiv : the preprint server for biology · 2023Article
- Bile acid fitness determinants of abioRxiv : the preprint server for biology · 2023Article
- What's the defect? Using mass defects to study oligomerization of membrane proteins and peptides in nanodiscs with native mass spectrometry.Methods (San Diego, Calif.) · 2023Review
- Structural mass spectrometry approaches to understand multidrug efflux systems.Essays in biochemistry · 2023Review
- Structural basis for lipid and copper regulation of the ABC transporter MsbA.Nature communications · 2022Article
- High-throughput functional characterization of protein phosphorylation sites in yeast.Nature biotechnology · 2022Article
- Lipid Head Group Adduction to Soluble Proteins Follows Gas-Phase Basicity Predictions: Dissociation Barriers and Charge Abstraction.International journal of mass spectrometry · 2021Article
- Potential Antioxidant and Anti-Inflammatory Function ofInternational journal of molecular sciences · 2021Article
- Exploring Phosphoinositide Binding Using Native Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The importance of the membrane for biophysical measurements.Nature chemical biology · 2020Review
- Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.Molecular cell · 2020Article
69 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
The ability of electrospray to propel large viruses into a mass spectrometer is established and is rationalized by analogy to the atmospheric transmission of the common cold. Much less clear is the fate of membrane-embedded molecular machines in the gas phase. Here we show that rotary adenosine triphosphatases (ATPases)/synthases from Thermus thermophilus and Enterococcus hirae can be maintained intact with membrane and soluble subunit interactions preserved in vacuum. Mass spectra reveal subunit stoichiometries and the identity of tightly bound lipids within the membrane rotors. Moreover, subcomplexes formed in solution and gas phases reveal the regulatory effects of nucleotide binding on both ATP hydrolysis and proton translocation. Consequently, we can link specific lipid and nucleotide binding with distinct regulatory roles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.