ArticleJournal of experimental botany2012
Arabidopsis SUC1 loads the phloem in suc2 mutants when expressed from the SUC2 promoter.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 44 citations in OpenAlex.
- The loss of COP1 delays senescence and inhibits root growth through modulation of sucrose allocation and availability.Plant physiology · 2026Article
- PLASMODESMATA-LOCATED PROTEIN 6 regulates plasmodesmal function in Arabidopsis vasculature.The Plant cell · 2024Article
- Light regulates widespread plant alternative polyadenylation through the chloroplast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structure and sucrose binding mechanism of the plant SUC1 sucrose transporter.Nature plants · 2023Article
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- Light regulates alternative splicing outcomes via the TOR kinase pathway.Cell reports · 2021Article
- Potassium Application Boosts Photosynthesis and Sorbitol Biosynthesis and Accelerates Cold Acclimation of Common Plantain (Plants (Basel, Switzerland) · 2020Article
- Suppressing a Putative Sterol Carrier Gene Reduces Plasmodesmal Permeability and Activates Sucrose Transporter Genes during Cotton Fiber Elongation.The Plant cell · 2017Article
- MYB76 Inhibits Seed Fatty Acid Accumulation in Arabidopsis.Frontiers in plant science · 2017Article
- Photosynthesis Activates Plasma Membrane H+-ATPase via Sugar Accumulation.Plant physiology · 2016Article
- Multispectral phloem-mobile probes: properties and applications.Plant physiology · 2015Article
- Transgenic approaches to altering carbon and nitrogen partitioning in whole plants: assessing the potential to improve crop yields and nutritional quality.Frontiers in plant science · 2015Review
- Article
- Bayesian phylogeny of sucrose transporters: ancient origins, differential expansion and convergent evolution in monocots and dicots.Frontiers in plant science · 2014Article
- Identification of MFS proteins in sorghum using semantic similarity.Theory in biosciences = Theorie in den Biowissenschaften · 2013Article
- Role of metabolite transporters in source-sink carbon allocation.Frontiers in plant science · 2013Article
- Structural and functional heterogeneity in phloem loading and transport.Frontiers in plant science · 2013Article
- Arg188 in rice sucrose transporter OsSUT1 is crucial for substrate transport.BMC biochemistry · 2012Article
- Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.Plant physiology · 2012Article
- Ustilago maydis: dissecting the molecular interface between pathogen and plant.PLoS pathogens · 2012Review
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Active loading of sucrose into phloem companion cells (CCs) is an essential process in apoplastic loaders, such as Arabidopsis or tobacco (Nicotiana sp.), and is even used by symplastic loaders such as melon (Cucumis melo) under certain stress conditions. Reduction of the amount or complete removal of the transporters catalysing this transport step results in severe developmental defects. Here we present analyses of two Arabidopsis lines, suc2-4 and suc2-5, that carry a null allele of the SUC2 gene which encodes the Arabidopsis phloem loader. These lines were complemented with constructs expressing either the Arabidopsis SUC1 or the Ustilago maydis srt1 cDNA from the SUC2 promoter. Both SUC1 and Srt1 are energy-dependent sucrose/H(+) symporters and differ in specific kinetic properties from the SUC2 protein. Transgene expression was confirmed by RT-PCRs, the subcellular localization of Srt1 in planta with an Srt1-RFP fusion, and the correct CC-specific localization of the recombinant proteins by immunolocalization with anti-Srt1 and anti-SUC1 antisera. The transport capacity of Srt1 was studied in Srt1-GFP expressing Arabidopsis protoplasts. Although both proteins were found exclusively in CCs, only SUC1 complemented the developmental defects of suc2-4 and suc2-5 mutants. As SUC1 and Srt1 are well characterized, this result provides an insight into the properties that are essential for sucrose transporters to load the phloem successfully.
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