ArticleProceedings of the National Academy of Sciences of the United States of America2011
Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Anatomy and Function of Deleted in Azoospermia Like (DAZL) Gene in Human and Mouse.Development & reproduction · 2025Article
- Article
- DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity.Nucleic acids research · 2022Article
- RBM22, a Key Player of Pre-mRNA Splicing and Gene Expression Regulation, Is Altered in Cancer.Cancers · 2022Review
- Review
- Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs.Nucleic acids research · 2021Article
- Direct reprogramming of human Sertoli cells into male germline stem cells with the self-renewal and differentiation potentials via overexpressing DAZL/DAZ2/BOULE genes.Stem cell reports · 2021Article
- Article
- Article
- The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation.Nature communications · 2020Article
- Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.PLoS genetics · 2020Article
- Dazl determines primordial follicle formation through the translational regulation of Tex14.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- DAZL is a master translational regulator of murine spermatogenesis.National science review · 2019Article
- DAZL Regulates Germ Cell Survival through a Network of PolyA-Proximal mRNA Interactions.Cell reports · 2018Article
- Requirement of the 3'-UTR-dependent suppression of DAZL in oocytes for pre-implantation mouse development.PLoS genetics · 2018Article
- In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells.Nature communications · 2017Article
- RNA-binding proteins in human oogenesis: Balancing differentiation and self-renewal in the female fetal germline.Stem cell research · 2017Review
- RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.Molecular human reproduction · 2017Article
- BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.Nature communications · 2017Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
A defect in germ-cell (sperm and oocyte) development is the leading cause of male and female infertility. Control of translation through the binding of deleted in azoospermia (DAZ)-like (DAZL) to the 3'-UTRs of mRNAs, via a highly conserved RNA recognition motif (RRM), has been shown to be essential in germ-cell development. Crystal structures of the RRM from murine DAZL (Dazl), both alone and in complex with RNA sequences from the 3'-UTRs of mRNAs regulated by Dazl, reveal high-affinity sequence-specific recognition of a GUU triplet involving an extended, kinked, pair of β-strands. Recognition of the GUU triplet is maintained, whereas the identity and position of bases flanking this triplet varies. The Dazl RRM is thus able to recognize GUU triplets in different sequence contexts. Mutation of bases within the GUU triplet reduces the affinity of binding. Together with the demonstration that multiple Dazl RRMs can bind to a single RNA containing multiple GUU triplets, these structures suggest that the number of DAZL molecules bound to GUU triplets in the 3'-UTR provides a method for modulating the translation of a target RNA. The conservation of RNA binding and structurally important residues between members of the DAZ family, together with the demonstration that mutation of these residues severely impairs RNA binding, indicate that the mode of RNA binding revealed by these structures is conserved in proteins essential for gamete development from flies to humans.
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