ArticleEukaryotic cell2012
SPM1 stabilizes subpellicular microtubules in Toxoplasma gondii.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 69 citations in OpenAlex.
- TgJosephin and TgRad23 are important for anti-IFN-γ virulence via deubiquitination of SPM1 inmSphere · 2026Article
- Microtubule inner proteins in apicomplexan parasites.Biochemical Society transactions · 2026Review
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- Atomic models of the Toxoplasma cell invasion machinery.Nature structural & molecular biology · 2026Article
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- Co-dependent formation of themBio · 2025Article
- Function and interactions of a protein bridge between the inner membrane complex and subpellicular microtubules inMolecular biology of the cell · 2025Article
- Deletion of splicing factor Cdc5 in Toxoplasma disrupts transcriptome integrity, induces abortive bradyzoite formation, and prevents acute infection in mice.Nature communications · 2025Article
- Microtubule inner proteins ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Luminal acetylation of microtubules is not essential forMicrobial cell (Graz, Austria) · 2025Article
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii.PLoS pathogens · 2024Article
- SPARK regulates AGC kinases central to theeLife · 2024Article
- Co-dependent formation of thebioRxiv : the preprint server for biology · 2024Article
- SPARK regulates AGC kinases central to thebioRxiv : the preprint server for biology · 2024Article
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance inbioRxiv : the preprint server for biology · 2024Article
- The initiation and early development of the tubulin-containing cytoskeleton in the human parasiteMolecular biology of the cell · 2024Article
- Balancing Act: Tubulin Glutamylation and Microtubule Dynamics inMicroorganisms · 2024Review
- The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement.Journal of cell science · 2023Article
- The protein phosphatase PPKL is a key regulator of daughter parasite development inbioRxiv : the preprint server for biology · 2023Article
- The Tubulin Superfamily in Apicomplexan Parasites.Microorganisms · 2023Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have identified two novel proteins that colocalize with the subpellicular microtubules in the protozoan parasite Toxoplasma gondii and named these proteins SPM1 and SPM2. These proteins have basic isoelectric points and both have homologs in other apicomplexan parasites. SPM1 contains six tandem copies of a 32-amino-acid repeat, whereas SPM2 lacks defined protein signatures. Alignment of Toxoplasma SPM2 with apparent Plasmodium SPM2 homologs indicates that the greatest degree of conservation lies in the carboxy-terminal half of the protein. Analysis of Plasmodium homologs of SPM1 indicates that while the central 32-amino-acid repeats have expanded to different degrees (7, 8, 9, 12, or 13 repeats), the amino- and carboxy-terminal regions remain conserved. In contrast, although the Cryptosporidium SPM1 homolog has a conserved carboxy tail, the five repeats are considerably diverged, and it has a smaller amino-terminal domain. SPM1 is localized along the full length of the subpellicular microtubules but does not associate with the conoid or spindle microtubules. SPM2 has a restricted localization along the middle region of the subpellicular microtubules. Domain deletion analysis indicates that four or more copies of the SPM1 repeat are required for localization to microtubules, and the amino-terminal 63 residues of SPM2 are required for localization to the subpellicular microtubules. Gene deletion studies indicate that neither SPM1 nor SPM2 is essential for tachyzoite viability. However, loss of SPM1 decreases overall parasite fitness and eliminates the stability of subpellicular microtubules to detergent extraction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.