ArticleJournal of bacteriology2012
The RNA chaperone Hfq independently coordinates expression of the VirB type IV secretion system and the LuxR-type regulator BabR in Brucella abortus 2308.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 54 citations in OpenAlex.
- Hfq modulates system-wide regulatory networks controlling biocontrol property inSynthetic and systems biotechnology · 2026Article
- The atypical quorum sensing system of classicalJournal of bacteriology · 2025Review
- Pathogenicity and virulence ofVirulence · 2025Review
- Quorum sensing LuxR proteins VjbR and BabR jointly regulateJournal of bacteriology · 2025Article
- ThemicroLife · 2025Review
- Post-transcriptional control of the essential enzyme MurF by a small regulatory RNA in Brucella abortus.Molecular microbiology · 2024Article
- AMicroorganisms · 2023Article
- TheFrontiers in microbiology · 2023Article
- Lysine Acylation Modification Landscape ofFrontiers in cell and developmental biology · 2022Article
- The VirB System Plays a Crucial Role inInternational journal of molecular sciences · 2021Review
- An Extracytoplasmic Function Sigma/Anti-Sigma Factor System Regulates Hypochlorous Acid Resistance and Impacts Expression of the Type IV Secretion System inJournal of bacteriology · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Different Impacts of MucR Binding to theBiomolecules · 2020Article
- Riboregulation in Nitrogen-Fixing Endosymbiotic Bacteria.Microorganisms · 2020Review
- Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing.Nucleic acids research · 2020Article
- Proteomic Analysis of Stationary Growth Stage Adaptation and Nutritional Deficiency Response ofFrontiers in microbiology · 2020Article
- The Cation Diffusion Facilitator Family Protein EmfA Confers Resistance to Manganese Toxicity in Brucella abortus 2308 and Is an Essential Virulence Determinant in Mice.Journal of bacteriology · 2019Article
- Comparative Transcriptome Analysis of Artificially Induced Rough-MutantFrontiers in veterinary science · 2019Article
- The Manganese-Dependent Pyruvate Kinase PykM Is Required for Wild-Type Glucose Utilization by Brucella abortus 2308 and Its Virulence in C57BL/6 Mice.Journal of bacteriology · 2018Article
- Characterization of Three Small Proteins in Brucella abortus Linked to Fucose Utilization.Journal of bacteriology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The type IV secretion system encoded by the virB operon is required for full virulence of Brucella sp., and the present study links the RNA chaperone Hfq to wild-type expression of virB in Brucella abortus 2308. Studies employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showed that both transcription and translation of virB are decreased in an isogenic hfq mutant compared to those in the parental strain. These results led to the hypothesis that Hfq regulation of virB is mediated through an intermediate transcriptional regulator. Subsequent experiments determined that expression of the gene encoding the putative Brucella quorum-sensing regulator BabR (also known as BlxR), a known virB regulator, is also controlled by Hfq at the posttranscriptional level, and a cis-acting element in the 5' untranslated region of the babR transcript responsible for this regulation was identified. Consistent with its role as a virB regulator, recombinant Brucella BabR binds to the virB promoter region in electrophoretic mobility shift assays. However, experiments employing a babR mutant strain determined that BabR is a repressor, not an activator, of virB transcription. These findings suggest that Hfq regulates virB expression through both BabR-dependent and BabR-independent pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.