ArticleApplied and environmental microbiology2012
Experimental evolution of a facultative thermophile from a mesophilic ancestor.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- The Maximum Growth Temperature for Eukaryotes Is Thermodynamically Driven but Ecologically Contingent.Life (Basel, Switzerland) · 2026Article
- Toward a systems metabolic engineering approach to improve thermotolerance of Escherichia coli.Microbial cell factories · 2026Review
- Enhancing the heat tolerance of homolactic Escherichia coli through reverse metabolic engineering.Applied microbiology and biotechnology · 2026Article
- Advances in adaptive laboratory evolution applications forSynthetic and systems biotechnology · 2025Review
- Characterization of a Novel RNA Polymerase Mutant of Escherichia coli That Confers Thermotolerance and Chaperone Independence.Molecular microbiology · 2025Article
- Constraints in temperature adaptation reinforce differences in thermal niche between mesophilic and psychrotolerantProceedings. Biological sciences · 2025Article
- Thermally adapted Escherichia coli keeps transcriptomic response during temperature upshift exposure.Applied microbiology and biotechnology · 2025Article
- Limited Diversity of Thermal Adaptation to a Critical Temperature inInternational journal of molecular sciences · 2025Article
- Bacterial Growth Temperature as a Horizontally Acquired Polygenic Trait.Genome biology and evolution · 2025Article
- Simultaneous saccharification and fermentation for D-lactic acid production using a metabolically engineered Escherichia coli adapted to high temperature.Biotechnology for biofuels and bioproducts · 2024Article
- Untangling the adaptive strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1 under low temperature.Extremophiles : life under extreme conditions · 2024Article
- Article
- Microbe-aided thermophilic composting accelerates manure fermentation.Frontiers in microbiology · 2024Article
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Article
- Adaptive laboratory evolution of a thermophile toward a reduced growth temperature optimum.Frontiers in microbiology · 2023Article
- Latent functional diversity may accelerate microbial community responses to temperature fluctuations.eLife · 2022Article
- Genomic attributes of thermophilic and hyperthermophilic bacteria and archaea.World journal of microbiology & biotechnology · 2022Review
- The Molecular Determinants of Thermoadaptation: Methanococcales as a Case Study.Molecular biology and evolution · 2021Article
- Evolution of Protein Structure and Stability in Global Warming.International journal of molecular sciences · 2020Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experimental evolution via continuous culture is a powerful approach to the alteration of complex phenotypes, such as optimal/maximal growth temperatures. The benefit of this approach is that phenotypic selection is tied to growth rate, allowing the production of optimized strains. Herein, we demonstrate the use of a recently described long-term culture apparatus called the Evolugator for the generation of a thermophilic descendant from a mesophilic ancestor (Escherichia coli MG1655). In addition, we used whole-genome sequencing of sequentially isolated strains throughout the thermal adaptation process to characterize the evolutionary history of the resultant genotype, identifying 31 genetic alterations that may contribute to thermotolerance, although some of these mutations may be adaptive for off-target environmental parameters, such as rich medium. We undertook preliminary phenotypic analysis of mutations identified in the glpF and fabA genes. Deletion of glpF in a mesophilic wild-type background conferred significantly improved growth rates in the 43-to-48°C temperature range and altered optimal growth temperature from 37°C to 43°C. In addition, transforming our evolved thermotolerant strain (EVG1064) with a wild-type allele of glpF reduced fitness at high temperatures. On the other hand, the mutation in fabA predictably increased the degree of saturation in membrane lipids, which is a known adaptation to elevated temperature. However, transforming EVG1064 with a wild-type fabA allele had only modest effects on fitness at intermediate temperatures. The Evolugator is fully automated and demonstrates the potential to accelerate the selection for complex traits by experimental evolution and significantly decrease development time for new industrial strains.
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