ReviewIntegrative biology : quantitative biosciences from nano to macro2012
Single-molecule studies of nucleocytoplasmic transport: from one dimension to three dimensions.
Review in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics.Chemical & biomedical imaging · 2023Review
- Unveiling the complexity: assessing models describing the structure and function of the nuclear pore complex.Frontiers in cell and developmental biology · 2023Review
- Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation.Nature protocols · 2021Article
- Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation.Biophysical reviews · 2019Review
- Single event visualization of unconventional secretion of FGF2.The Journal of cell biology · 2019Article
- Nuclear export of mRNA molecules studied by SPEED microscopy.Methods (San Diego, Calif.) · 2019Review
- Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors.Plant physiology · 2016Article
- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Nanoscale stiffness topography reveals structure and mechanics of the transport barrier in intact nuclear pore complexes.Nature nanotechnology · 2015Article
- Review
- High-resolution imaging reveals new features of nuclear export of mRNA through the nuclear pore complexes.International journal of molecular sciences · 2014Review
- Hamiltonian Switch Metropolis Monte Carlo Simulations for Improved Conformational Sampling of Intrinsically Disordered Regions Tethered to Ordered Domains of Proteins.Journal of chemical theory and computation · 2014Article
- Bending the rules: widefield microscopy and the Abbe limit of resolution.Journal of cellular physiology · 2014Review
- Role of molecular charge in nucleocytoplasmic transport.PloS one · 2014Article
- Model inspired by nuclear pore complex suggests possible roles for nuclear transport receptors in determining its structure.Biophysical journal · 2013Article
- High-resolution three-dimensional mapping of mRNA export through the nuclear pore.Nature communications · 2013Article
- Nuclear pore complex composition: a new regulator of tissue-specific and developmental functions.Nature reviews. Molecular cell biology · 2012Review
- Distinct, but not completely separate spatial transport routes in the nuclear pore complex.Nucleus (Austin, Tex.)Review
- A single molecule view on Dbp5 and mRNA at the nuclear pore.Nucleus (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In eukaryotic cells, the bidirectional trafficking of proteins and genetic materials across the double-membrane nuclear envelope is mediated by nuclear pore complexes (NPCs). A highly selective barrier formed by the phenylalanine-glycine (FG)-nucleoporin (Nup) in the NPC allows for two transport modes: passive diffusion and transport receptor-facilitated translocation. Strict regulation of nucleocytoplasmic transport is crucial for cell survival, differentiation, growth and other essential activities. However, due to the limited knowledge of the native configuration of the FG-Nup barrier and the interactions between the transiting molecules and the barrier in the NPC, the precise nucleocytoplasmic transport mechanism remains unresolved. To refine the transport mechanism, single-molecule fluorescence microscopy methods have been employed to obtain the transport kinetics of individual fluorescent molecules through the NPC and to map the interactions between transiting molecules and the FG-Nup barrier. Important characteristics of nucleocytoplasmic transport, such as transport time, transport efficiency and spatial distribution of single transiting molecules in the NPC, have been obtained that could not be measured by either ensemble average methods or conventional electron microscopy. In this critical review, we discuss the development of various single-molecule techniques and their application to nucleocytoplasmic transport in vitro and in vivo. In particular, we highlight a recent advance from one-dimensional to three-dimensional single-molecule characterization of transport through the NPC and present a comprehensive understanding of the nucleocytoplasmic transport mechanism obtained by this new technical development (105 references).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.