ArticleNature methods2011
A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans.Stem cell reports · 2025Article
- A newly identified gene Ahed plays essential roles in murine haematopoiesis.Nature communications · 2024Article
- Comparison of CRISPR-Cas9-mediated megabase-scale genome deletion methods in mouse embryonic stem cells.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
- Cooperative genetic networks drive embryonic stem cell transition from naïve to formative pluripotency.The EMBO journal · 2021Article
- Overcoming off-targets: assessing Western blot signals for Bcnt/Cfdp1, a tentative component of the chromatin remodeling complex.Bioscience reports · 2020Article
- Generation of targeted homozygosity in the genome of human induced pluripotent stem cells.PloS one · 2019Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
- Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.Nucleic acids research · 2018Article
- Identification of a Golgi GPI-N-acetylgalactosamine transferase with tandem transmembrane regions in the catalytic domain.Nature communications · 2018Article
- Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens.Nucleic acids research · 2017Article
- An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.Nucleic acids research · 2017Article
- NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation.RNA (New York, N.Y.) · 2017Article
- Genome-Wide Screening Uncovers the Significance of N-Sulfation of Heparan Sulfate as a Host Cell Factor for Chikungunya Virus Infection.Journal of virology · 2017Article
- Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins.Scientific reports · 2017Article
- Chromatin states shape insertion profiles of the piggyBac, Tol2 and Sleeping Beauty transposons and murine leukemia virus.Scientific reports · 2017Article
- Post-Golgi anterograde transport requires GARP-dependent endosome-to-TGN retrograde transport.Molecular biology of the cell · 2015Article
- Genome-Wide Screening of Genes Required for Glycosylphosphatidylinositol Biosynthesis.PloS one · 2015Article
- Application of an inducible transposon with anther culture in generation of di-haploid homologous mutants.Botanical studies · 2014Article
- Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells.BMC genomics · 2014Article
- Rapid selection of XO embryonic stem cells using Y chromosome-linked GFP transgenic mice.Transgenic research · 2014Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Genome-wide mutagenesis in mouse embryonic stem cells (ESCs) is a powerful tool, but the diploid nature of the mammalian genome hampers its application for recessive genetic screening. We have previously reported a method to induce homozygous mutant ESCs from heterozygous mutants by tetracycline-dependent transient disruption of the Bloom's syndrome gene. However, we could not purify homozygous mutants from a large population of heterozygous mutant cells, limiting the applications. Here we developed a strategy for rapid enrichment of homozygous mutant mouse ESCs and demonstrated its feasibility for cell-based phenotypic analysis. The method uses G418-plus-puromycin double selection to enrich for homozygotes and single-nucleotide polymorphism analysis for identification of homozygosity. We combined this simple approach with gene-trap mutagenesis to construct a homozygous mutant ESC bank with 138 mutant lines and demonstrate its use in phenotype-driven genetic screening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.