ArticleActa biomaterialia2012
Controlled release of vascular endothelial growth factor using poly-lactic-co-glycolic acid microspheres: in vitro characterization and application in polycaprolactone fumarate nerve conduits.
Article in Acta biomaterialia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Prevascularization of electrospun PCL/PLA scaffolds using human adipose-derived stem and endothelial cells enhances vascular integration and host angiogenesis in vivo.Stem cell research & therapy · 2026Article
- Recent Advances in the Preparation of Protein/peptide Microspheres by Solvent Evaporation Method.Current pharmaceutical biotechnology · 2024Review
- Injectable neural hydrogel asRegenerative engineering and translational medicine · 2023Article
- Recent Developments in Biopolymer-Based Hydrogels for Tissue Engineering Applications.Biomolecules · 2023Review
- A Protein Composite Neural Scaffold Modulates Astrocyte Migration and Transcriptome Profile.Macromolecular bioscience · 2022Article
- The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Newly regenerated axons via scaffolds promote sub-lesional reorganization and motor recovery with epidural electrical stimulation.NPJ Regenerative medicine · 2021Article
- Recent Progress on Polysaccharide-Based Hydrogels for Controlled Delivery of Therapeutic Biomolecules.ACS biomaterials science & engineering · 2021Review
- Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration.Scientific reports · 2021Article
- Theranostic Designed Near-Infrared Fluorescent Poly (Lactic-co-Glycolic Acid) Nanoparticles and Preliminary Studies with Functionalized VEGF-Nanoparticles.Journal of clinical medicine · 2020Article
- Development of a nanomedicine-loaded hydrogel for sustained delivery of an angiogenic growth factor to the ischaemic myocardium.Drug delivery and translational research · 2020Article
- Evaluation of recombinant human vascular endothelial growth factor VEGF121-loaded poly-l-lactide microparticles as a controlled release delivery system.Turkish journal of biology = Turk biyoloji dergisi · 2020Article
- [Advance of vascularization of tissue engineered peripheral nerve].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019Article
- Sustained Release of Vascular Endothelial Growth Factor from Poly(ε-caprolactone-PEG-ε-caprolactone)-ACS omega · 2019Article
- Endogenous automatic nerve discharge promotes nerve repair: an optimized animal model.Neural regeneration research · 2019Article
- Tissue Engineering in Pediatric Bladder Reconstruction-The Road to Success.Frontiers in pediatrics · 2019Review
- Injectable shear-thinning hydrogels for delivering osteogenic and angiogenic cells and growth factors.Biomaterials science · 2018Article
- Phrenic and intercostal nerves with rhythmic discharge can promote early nerve regeneration after brachial plexus repair in rats.Neural regeneration research · 2018Article
- Role of Hippo Pathway Effector Tafazzin Protein in Maintaining Stemness of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC).International journal of hematology-oncology and stem cell research · 2018Article
- Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator. Controlled release of such stimulators may enhance and guide the vascularization process, and when applied in a nerve conduit may play a role in nerve regeneration. We report the fabrication and in vitro characterization of poly-lactic-co-glycolic acid (PLGA) microspheres encapsulating VEGF and the in vivo application of nerve conduits supplemented with VEGF-containing microspheres. PLGA microspheres containing VEGF were prepared by the double emulsion-solvent evaporation technique. This yielded 83.16% of microspheres with a diameter <53 μm. VEGF content measured by ELISA indicated 93.79±10.64% encapsulation efficiency. Release kinetics were characterized by an initial burst release of 67.6±8.25% within the first 24h, followed by consistent release of approximately 0.34% per day for 4 weeks. Bioactivity of the released VEGF was tested by human umbilical vein endothelial cell (HUVEC) proliferation assay. VEGF released at all time points enhanced HUVEC proliferation, confirming that VEGF retained its bioactivity throughout the 4 week time period. When the microsphere delivery system was placed in a biosynthetic nerve scaffold robust nerve regeneration was observed. This study established a novel system for controlled release of growth factors and enables in vivo studies of nerve conduits conditioned with this system.
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