ArticleJournal of immunological methods2012
The development of a sensitive and specific ELISA for mouse eosinophil peroxidase: assessment of eosinophil degranulation ex vivo and in models of human disease.
Article in Journal of immunological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF.Journal of leukocyte biology · 2020Trial
- Biomarkers of Type 2 Airway Inflammation as Predictors of Loss of Asthma Control During Step-Down Therapy for Well-Controlled Disease: The Long-Acting Beta-Agonist Step-Down Study (LASST).The journal of allergy and clinical immunology. In practiceTrial
- Activation of donor-derived eosinophils during static storage contributes to primary graft dysfunction after lung transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2026Article
- Topical application of the HSP90 inhibitor 17-AAG reduces skin inflammation and partially restores microbial balance: implications for atopic dermatitis therapy.Scientific reports · 2025Article
- TLR7-dependent eosinophil degranulation links psoriatic skin inflammation to small intestinal inflammatory changes in mice.Experimental & molecular medicine · 2024Article
- Assessment of Lung Eosinophils In Situ Using Immunohistological Staining.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Nasal eosinophilia and eosinophil peroxidase in children and adolescents with rhinitis.Korean journal of pediatrics · 2019Article
- Charcot-Leyden crystal protein/galectin-10 is a surrogate biomarker of eosinophilic airway inflammation in asthma.Biomarkers in medicine · 2019Article
- Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation.The Journal of experimental medicine · 2018Article
- Revisiting the NIH Taskforce on the Research needs of Eosinophil-Associated Diseases (RE-TREAD).Journal of leukocyte biology · 2018Review
- Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.Communications biology · 2018Article
- Lung Pathologies in a Chronic Inflammation Mouse Model Are Independent of Eosinophil Degranulation.American journal of respiratory and critical care medicine · 2017Article
- Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nasal and pharyngeal eosinophil peroxidase levels in adults with poorly controlled asthma correlate with sputum eosinophilia.Allergy · 2016Article
- Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge.The Journal of allergy and clinical immunology · 2015Article
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- Eosinophils are important for protection, immunoregulation and pathology during infection with nematode microfilariae.PLoS pathogens · 2014Article
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- Eosinophil cytokines, chemokines, and growth factors: emerging roles in immunity.Frontiers in immunology · 2014Review
- Cys-leukotrienes promote fibrosis in a mouse model of eosinophil-mediated respiratory inflammation.American journal of respiratory cell and molecular biology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Mouse models of eosinophilic disorders are often part of preclinical studies investigating the underlying biological mechanisms of disease pathology. The presence of extracellular eosinophil granule proteins in affected tissues is a well established and specific marker of eosinophil activation in both patients and mouse models of human disease. Unfortunately, assessments of granule proteins in the mouse have been limited by the availability of specific antibodies and a reliance on assays of released enzymatic activities that are often neither sensitive nor eosinophil specific. The ability to detect immunologically and quantify the presence of a mouse eosinophil granule protein in biological fluids and/or tissue extracts was achieved by the generation of monoclonal antibodies specific for eosinophil peroxidase (EPX). This strategy identified unique pairs of antibodies with high avidity to the target protein and led to the development of a unique sandwich ELISA for the detection of EPX. Full factorial design was used to develop this ELISA, generating an assay that is eosinophil-specific and nearly 10 times more sensitive than traditional OPD-based detection methods of peroxidase activity. The added sensitivity afforded by this novel assay was used to detect and quantify eosinophil degranulation in several settings, including bronchoalveolar fluid from OVA sensitized/challenged mice (an animal model of asthma), serum samples derived from peripheral blood recovered from the tail vasculature, and from purified mouse eosinophils stimulated ex vivo with platelet activating factor (PAF) and PAF + ionomycin. This ability to assess mouse eosinophil degranulation represents a specific, sensitive, and reproducible assay that fulfills a critical need in studies of eosinophil-associated pathologies in mice.
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