ArticleJournal of molecular biology2011
Structure of the Escherichia coli phosphonate binding protein PhnD and rationally optimized phosphonate biosensors.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- A Portable Fluorometer for the Detection of Glyphosate.Biosensors · 2026Article
- Structural basis and evolutionary pathways of glycerol-1-phosphate transport in marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid Trafficking in Diverse Bacteria.Accounts of chemical research · 2025Review
- Computational design of Periplasmic binding protein biosensors guided by molecular dynamics.PLoS computational biology · 2024Article
- Novel sterol binding domains in bacteria.eLife · 2024Article
- Transcriptomic Insights into Archaeal Nitrification in the Amundsen Sea Polynya, Antarctica.Journal of microbiology (Seoul, Korea) · 2023Article
- The Microbial Degradation of Natural and Anthropogenic Phosphonates.Molecules (Basel, Switzerland) · 2023Review
- Marine picocyanobacterial PhnD1 shows specificity for various phosphorus sources but likely represents a constitutive inorganic phosphate transporter.The ISME journal · 2023Article
- Article
- ProX from marine Synechococcus spp. show a sole preference for glycine-betaine with differential affinity between ecotypes.Environmental microbiology · 2022Article
- Structural dynamics in the evolution of a bilobed protein scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle.Nature communications · 2021Article
- Structure- and mechanism-guided design of single fluorescent protein-based biosensors.Nature chemical biology · 2021Review
- Spontaneous Phage Resistance in Avian PathogenicFrontiers in microbiology · 2021Article
- Article
- Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events.Current opinion in cell biology · 2020Review
- Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.International journal of molecular sciences · 2019Review
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Quantifying the Intrinsic Conformation Energy Landscape Topography of Proteins with Large-Scale Open-Closed Transition.ACS central science · 2018Article
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Authors and funding
6 authors.
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Abstract
The phnD gene of Escherichia coli encodes the periplasmic binding protein of the phosphonate (Pn) uptake and utilization pathway. We have crystallized and determined structures of E. coli PhnD (EcPhnD) in the absence of ligand and in complex with the environmentally abundant 2-aminoethylphosphonate (2AEP). Similar to other bacterial periplasmic binding proteins, 2AEP binds near the center of mass of EcPhnD in a cleft formed between two lobes. Comparison of the open, unliganded structure with the closed 2AEP-bound structure shows that the two lobes pivot around a hinge by ~70° between the two states. Extensive hydrogen bonding and electrostatic interactions stabilize 2AEP, which binds to EcPhnD with low nanomolar affinity. These structures provide insight into Pn uptake by bacteria and facilitated the rational design of high signal-to-noise Pn biosensors based on both coupled small-molecule dyes and autocatalytic fluorescent proteins.
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