ArticleCurrent biology : CB2011
Rapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryos.
Article in Current biology : CB, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 46 citations in OpenAlex.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- FLInt 2.0: robust and customizable single-shot integration in C. elegans.G3 (Bethesda, Md.) · 2026Article
- Increased maize chromosome number by engineered chromosome fission.Science advances · 2025Article
- FAN1-mediated translesion synthesis and POLQ/HELQ-mediated end joining generate interstrand crosslink-induced mutations.Nature communications · 2025Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Nematode chromosomes.Genetics · 2022Review
- The ESCRT machinery directs quality control over inner nuclear membrane architecture.Cell reports · 2022Article
- Formation of artificial chromosomes in Caenorhabditis elegans and analyses of their segregation in mitosis, DNA sequence composition and holocentromere organization.Nucleic acids research · 2021Article
- RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos.Nucleic acids research · 2021Article
- Epigenetic regulation of centromere function.Cellular and molecular life sciences : CMLS · 2020Review
- Genetic and epigenetic effects on centromere establishment.Chromosoma · 2020Review
- TheGenetics · 2019Review
- Heterochromatin and RNAi regulate centromeres by protecting CENP-A from ubiquitin-mediated degradation.PLoS genetics · 2018Article
- Histone H3K9 and H4 Acetylations and Transcription Facilitate the Initial CENP-AEpigenetics & chromatin · 2018Article
- Reduction in chromosome mobility accompanies nuclear organization during early embryogenesis in Caenorhabditis elegans.Scientific reports · 2017Article
- Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Differential Chromosomal Localization of Centromeric Histone CENP-A Contributes to Nematode Programmed DNA Elimination.Cell reports · 2016Article
- Cell Size Determines the Strength of the Spindle Assembly Checkpoint during Embryonic Development.Developmental cell · 2016Article
- Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin.PLoS genetics · 2015Article
- Defining heterochromatin in C. elegans through genome-wide analysis of the heterochromatin protein 1 homolog HPL-2.Genome research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
DNA injected into the Caenorhabditis elegans germline forms extrachromosomal arrays that segregate during cell division [1, 2]. The mechanisms underlying array formation and segregation are not known. Here, we show that extrachromosomal arrays form de novo centromeres at high frequency, providing unique access to a process that occurs with extremely low frequency in other systems [3-8]. De novo centromerized arrays recruit centromeric chromatin and kinetochore proteins and autonomously segregate on the spindle. Live imaging following DNA injection revealed that arrays form after oocyte fertilization via homologous recombination and nonhomologous end-joining. Individual arrays gradually transition from passive inheritance to active segregation during the early embryonic divisions. The heterochromatin protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for de novo centromerization even though arrays become strongly enriched for the heterochromatin-associated H3K9me3 modification over time. Partial inhibition of HP1 family proteins accelerates the acquisition of segregation competence. In addition to reporting the first direct visualization of new centromere formation in living cells, these findings reveal that naked DNA rapidly builds de novo centromeres in C. elegans embryos in an HP1-independent manner and suggest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centromerization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.