ArticleMicrobial biotechnology2012
Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Novel utilization of the seaweed-based deoxy sugars rhamnose and fucose by engineered Corynebacterium glutamicum.Microbial cell factories · 2026Article
- Enhancement of ergothioneine production in Corynebacterium glutamicum by increasing osmotic pressure.Applied microbiology and biotechnology · 2025Article
- EngineeringBiodesign research · 2025Review
- Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.Microbial cell factories · 2024Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions.Applied microbiology and biotechnology · 2021Review
- Inducible Expression Systems Based on Xenogeneic Silencing and Counter-Silencing and Design of a Metabolic Toggle Switch.ACS synthetic biology · 2020Article
- Role of ClpB FromFrontiers in microbiology · 2020Article
- Catabolic Division of Labor Enhances Production of D-Lactate and Succinate From Glucose-Xylose Mixtures in EngineeredFrontiers in bioengineering and biotechnology · 2020Article
- Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineeredBiotechnology for biofuels · 2020Article
- Bromination of L-tryptophan in a Fermentative Process WithFrontiers in bioengineering and biotechnology · 2019Article
- Redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2017Article
- Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids.BMC genomics · 2017Article
- Engineered biosynthesis of biodegradable polymers.Journal of industrial microbiology & biotechnology · 2016Review
- Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli.BMC biotechnology · 2016Article
- Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model.Microbial cell factories · 2016Article
- Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.PloS one · 2016Article
- Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum.PloS one · 2016Article
- Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum.Microbial cell factories · 2015Article
- Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes.Microbial biotechnology · 2015Article
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4 authors.
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Abstract
Corynebacterium glutamicum, an established industrial amino acid producer, has been genetically modified for efficient succinate production from the renewable carbon source glucose under fully aerobic conditions in minimal medium. The initial deletion of the succinate dehydrogenase genes (sdhCAB) led to an accumulation of 4.7 g l(-1) (40 mM) succinate as well as high amounts of acetate (125 mM) as by-product. By deleting genes for all known acetate-producing pathways (pta-ackA, pqo and cat) acetate production could be strongly reduced by 83% and succinate production increased up to 7.8 g l(-1) (66 mM). Whereas overexpression of the glyoxylate shunt genes (aceA and aceB) or overproduction of the anaplerotic enzyme pyruvate carboxylase (PCx) had only minor effects on succinate production, simultaneous overproduction of pyruvate carboxylase and PEP carboxylase resulted in a strain that produced 9.7 g l(-1) (82 mM) succinate with a specific productivity of 1.60 mmol g (cdw)(-1) h(-1). This value represents the highest productivity among currently described aerobic bacterial succinate producers. Optimization of the production conditions by decoupling succinate production from cell growth using the most advanced producer strain (C. glutamicumΔpqoΔpta-ackAΔsdhCABΔcat/pAN6-pyc(P458S) ppc) led to an additional increase of the product yield to 0.45 mol succinate mol(-1) glucose and a titre of 10.6 g l(-1) (90 mM) succinate.
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