ArticleAnalytical chemistry2011
Aptamer-functionalized microgel particles for protein detection.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Recent Advances in Responsive Microgels for Biomedical Application.Bioengineering (Basel, Switzerland) · 2026Review
- Stimuli-Responsive Hydrogels: From Swelling-Deswelling Mechanisms to Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2026Review
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins.Nature communications · 2020Article
- Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels.Polymers · 2020Article
- DNA hydrogel-empowered biosensing.Advances in colloid and interface science · 2020Review
- Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels.Frontiers in medicine · 2020Review
- Nonfouling, Encoded Hydrogel Microparticles for Multiplex MicroRNA Profiling Directly from Formalin-Fixed, Paraffin-Embedded Tissue.Analytical chemistry · 2018Article
- Microparticle parking and isolation for highly sensitive microRNA detection.Lab on a chip · 2017Article
- Flexible Octopus-Shaped Hydrogel Particles for Specific Cell Capture.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles.Scientific reports · 2016Article
- Functional nucleic acid-based hydrogels for bioanalytical and biomedical applications.Chemical Society reviews · 2016Review
- Hydrogel microparticles for biosensing.European polymer journal · 2015Article
- An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system.Nature chemistry · 2015Article
- Gold-nanoparticle-decorated silica nanorods for sensitive visual detection of proteins.Analytical chemistry · 2014Article
- Micropatterned sensing hydrogels integrated with reconfigurable microfluidics for detecting protease release from cells.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Highly sensitive and multiplexed detection of clinically relevant proteins in biologically complex samples is crucial for the advancement of clinical proteomics. In recent years, aptamers have emerged as useful tools for protein analysis due to their specificity and affinity for protein targets as well as their compatibility with particle-based detection systems. In this study, we demonstrate the highly sensitive detection of human α-thrombin on encoded hydrogel microparticles functionalized with an aptamer capture sequence. We use static imaging and microfluidic flow-through analysis techniques to evaluate the detection capabilities of the microgels in sandwich-assay formats that utilize both aptamers and antibodies for the reporting of target-binding events. Buffers and reagent concentrations were optimized to provide maximum reaction efficiency while still maintaining an assay with a simple workflow that can be easily adapted to the multiplexed detection of other clinically relevant proteins. The three-dimensional, nonfouling hydrogel immobilization scaffold used in this work provides three logs of dynamic range, with a limit of detection of 4 pM using a single aptamer capture species and without the need for spacers or signal amplification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.