ArticlePloS one2011
Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers, 1 of them a synthesis that pooled it.
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Who cites it
120 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Send more data: a systematic review of mathematical models of antimicrobial resistance.Antimicrobial resistance and infection control · 2018Pooled it
- Metagenomics for antimicrobial resistance: from resistome surveillance to mechanistic inference.Journal of bacteriology · 2026Review
- Fecal sludge and adjacent drinking water as reservoirs of multidrug resistant ESBL-producing pathogenic Escherichia coli in Rohingya Camps, Bangladesh.Scientific reports · 2026Article
- Accurate plasmid reconstruction from metagenomics data using assembly-alignment graphs and contrastive learning.Nature biotechnology · 2026Article
- Longitudinal replicated metagenomic analysis of biosolids-amended soils reveals enrichment of ARGs, virulence factors, and ESKAPE pathogens.npj antimicrobials and resistance · 2025Article
- Article
- Towards the integration of antibiotic resistance gene mobility into environmental surveillance and risk assessment.npj antimicrobials and resistance · 2025Review
- Waste Sludge: Entirely Waste or a Sustainable Source of Biocrude? A Review.Applied biochemistry and biotechnology · 2024Review
- Article
- The CRISPR/Cas system as an antimicrobial resistance strategy in aquatic ecosystems.Functional & integrative genomics · 2024Review
- Potential probiotic Lactobacillus delbrueckii subsp. lactis KUMS-Y33 suppresses adipogenesis and promotes osteogenesis in human adipose-derived mesenchymal stem cell.Scientific reports · 2024Article
- Detecting horizontal gene transfer with metagenomics co-barcoding sequencing.Microbiology spectrum · 2024Article
- Emergence of Carbapenemase Genes in Gram-Negative Bacteria Isolated from the Wastewater Treatment Plant in A Coruña, Spain.Antibiotics (Basel, Switzerland) · 2024Article
- Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes.ISME communications · 2024Article
- Metagenomic insights into microbial community, functional annotation, and antibiotic resistance genes in Himalayan Brahmaputra River sediment, India.Frontiers in microbiology · 2024Article
- Isolation, molecular identification, and genomic analysis ofFrontiers in microbiology · 2024Article
- Multilayer networks of plasmid genetic similarity reveal potential pathways of gene transmission.The ISME journal · 2023Article
- Metagenomic profiling of antibiotic resistance genes in Red Sea brine pools.Archives of microbiology · 2023Article
- Integrons in the development of antimicrobial resistance: critical review and perspectives.Frontiers in microbiology · 2023Review
- Bacterial Resistance to β-Lactam Antibiotics in Municipal Wastewater: Insights from a Full-Scale Treatment Plant in Poland.Microorganisms · 2022Article
60 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.
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