ArticlePloS one2011
Uniaxial mechanical strain modulates the differentiation of neural crest stem cells into smooth muscle lineage on micropatterned surfaces.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Biomimetic scaffolds based on stem cells for neural tissue engineering: a review.Stem cell research & therapy · 2026Review
- From neural crest migration to the onset of gangliogenesis.Current topics in developmental biology · 2025Review
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
- An Easy-to-Fabricate Cell Stretcher Reveals Density-Dependent Mechanical Regulation of Collective Cell Movements in Epithelia.Cellular and molecular bioengineering · 2021Article
- Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation.Nano today · 2021Article
- Matrix stiffness modulates the differentiation of neural crest stem cells in vivo.Journal of cellular physiology · 2019Article
- Studying Human Neurological Disorders Using Induced Pluripotent Stem Cells: From 2D Monolayer to 3D Organoid and Blood Brain Barrier Models.Comprehensive physiology · 2019Review
- Adult Stem Cells in Vascular Remodeling.Theranostics · 2018Review
- Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues.PloS one · 2017Article
- Hyaluronan Is Crucial for Stem Cell Differentiation into Smooth Muscle Lineage.Stem cells (Dayton, Ohio) · 2016Article
- TRPM7 maintains progenitor-like features of neuroblastoma cells: implications for metastasis formation.Oncotarget · 2015Article
- Hedgehog and Resident Vascular Stem Cell Fate.Stem cells international · 2015Review
- Engineering of arteries in vitro.Cellular and molecular life sciences : CMLS · 2014Review
- Human iPSC-derived neural crest stem cells promote tendon repair in a rat patellar tendon window defect model.Tissue engineering. Part A · 2013Article
- Mechanobiology of human pluripotent stem cells.Tissue engineering. Part B, Reviews · 2013Review
- Effect of cyclic strain on cardiomyogenic differentiation of rat bone marrow derived mesenchymal stem cells.PloS one · 2012Article
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7 authors.
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Abstract
Neural crest stem cells (NCSCs) play an important role in the development and represent a valuable cell source for tissue engineering. However, how mechanical factors in vivo regulate NCSC differentiation is not understood. Here NCSCs were derived from induced pluripotent stem cells and used as a model to determine whether vascular mechanical strain modulates the differentiation of NCSCs into smooth muscle (SM) lineage. NCSCs were cultured on micropatterned membranes to mimic the organization of smooth muscle cells (SMCs), and subjected to cyclic uniaxial strain. Mechanical strain enhanced NCSC proliferation and ERK2 phosphorylation. In addition, mechanical strain induced contractile marker calponin-1 within 2 days and slightly induced SM myosin within 5 days. On the other hand, mechanical strain suppressed the differentiation of NCSCs into Schwann cells. The induction of calponin-1 by mechanical strain was inhibited by neural induction medium but further enhanced by TGF-β. For NCSCs pre-treated with TGF-β, mechanical strain induced the gene expression of both calponin-1 and SM myosin. Our results demonstrated that mechanical strain regulates the differentiation of NCSCs in a manner dependent on biochemical factors and the differentiation stage of NCSCs. Understanding the mechanical regulation of NCSC differentiation will shed light on the development and remodeling of vascular tissues, and how transplanted NCSCs respond to mechanical factors.
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