ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2012
Regulation of the gene encoding the intestinal bile acid transporter ASBT by the caudal-type homeobox proteins CDX1 and CDX2.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Altered Bile Acid Transport in Liver Disease.Biomedicines · 2026Review
- Novel Expression of Apical Bile Acid Transport (ASBT) More Proximally Than Distal Ileum Contributing to Enhanced Intestinal Bile Acid Absorption in Obesity.International journal of molecular sciences · 2024Article
- Deletion of Intestinal SHP Impairs Short-term Response to Cholic Acid Challenge in Male Mice.Endocrinology · 2021Article
- Caudal type homeoboxes as a driving force inGut microbes · 2020Article
- Intestinal Absorption of Bile Acids in Health and Disease.Comprehensive Physiology · 2019Review
- Organic Solute Transporter α-β Protects Ileal Enterocytes From Bile Acid-Induced Injury.Cellular and molecular gastroenterology and hepatology · 2018Article
- CDX2 upregulates SLC26A3 gene expression in intestinal epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Identification and validation of soluble carrier family expression signature for predicting poor outcome of renal cell carcinoma.Journal of Cancer · 2017Article
- Hepatocyte nuclear factor 1 coordinates multiple processes in a model of intestinal epithelial cell function.Biochimica et biophysica acta · 2016Article
- pH Dependent but not P-gp Dependent Bidirectional Transport Study of S-propranolol: The Importance of Passive Diffusion.Pharmaceutical research · 2015Article
- Extending the functions of the homeotic transcription factor Cdx2 in the digestive system through nontranscriptional activities.World journal of gastroenterology · 2015Review
- New insights into the functions and localization of the homeotic gene CDX2 in gastric cancer.World journal of gastroenterology · 2014Review
- Proton Pump Inhibitors: The Culprit for Barrett's Esophagus?Frontiers in oncology · 2014Review
- c-Fos mediates repression of the apical sodium-dependent bile acid transporter by fibroblast growth factor-19 in mice.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Reversal of multidrug resistance in gastric cancer cells by CDX2 downregulation.World journal of gastroenterology · 2013Article
- Relationships of CDXs and apical sodium-dependent bile acid transporter in Barrett's esophagus.World journal of gastroenterology · 2013Article
- Article
- The solute carrier family 10 (SLC10): beyond bile acid transport.Molecular aspects of medicineReview
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The apical sodium-dependent bile acid transporter (ASBT) is expressed abundantly in the ileum and mediates bile acid absorption across the apical membranes. Caudal-type homeobox proteins CDX1 and CDX2 are transcription factors that regulate genes involved in intestinal epithelial differentiation and proliferation. Aberrant expression of both ASBT and CDXs in Barrett's esophagus (BE) prompted us to study, whether the expression of the ASBT gene is regulated by CDXs. Short interfering RNA-mediated knockdown of CDXs resulted in reduced ASBT mRNA expression in intestinal cells. CDXs strongly induced the activity of the ASBT promoter in reporter assays in esophageal and intestinal cells. Nine CDX binding sites were predicted in silico within the ASBT promoter, and binding of CDXs to six of them was verified in vitro and within living cells by electrophoretic mobility shift assays and chromatin immunoprecipitation assays, respectively. RNAs were extracted from esophageal biopsies from 20 BE patients and analyzed by real-time PCR. Correlation with ASBT expression was found for CDX1, CDX2, and HNF-1α in BE biopsies. In conclusion, the human ASBT promoter is activated transcriptionally by CDX1 and CDX2. Our finding provides a possible explanation for the reported observation that ASBT is aberrantly expressed in esophageal metaplasia that also expresses CDX transcription factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.