ArticleHuman reproduction (Oxford, England)2011
Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions.
Article in Human reproduction (Oxford, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 82 citations in OpenAlex.
- Fertility preservation in prepubertal boys: management and follow-up challenges.Endocrine connections · 2026Review
- In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration.Clinical and experimental reproductive medicine · 2024Article
- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches.Frontiers in endocrinology · 2022Review
- The Role of MicroRNA 143 and MicroRNA 206 in The Regulation of Apoptosis in Mouse Lukemia Cancer Cells and Spermatogonial Cells.Cell journal · 2021Article
- Disease-inducing potential of two leukemic cell lines in a xenografting model.Journal of assisted reproduction and genetics · 2021Article
- Spermatogonial Stem Cell Culture in Oncofertility.The Urologic clinics of North America · 2020Review
- Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems.Biomolecules · 2020Review
- Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.Stem cell research & therapy · 2019Article
- Formation of organotypic testicular organoids in microwell culture†.Biology of reproduction · 2019Article
- Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019Article
- Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments.International journal of molecular sciences · 2018Review
- Cryostorage of immature and mature human testis tissue to preserve spermatogonial stem cells (SSCs): a systematic review of current experiences toward clinical applications.Stem cells and cloning : advances and applications · 2018Review
- Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro.International journal of nanomedicine · 2018Article
- Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells.Human reproduction (Oxford, England) · 2018Article
- Process and Pitfalls of Sperm Cryopreservation.Journal of clinical medicine · 2017Review
- Article
- Spermatogonial stem cell autotransplantation and germline genomic editing: a future cure for spermatogenic failure and prevention of transmission of genomic diseases.Human reproduction update · 2016Review
- Toward precision medicine for preserving fertility in cancer patients: existing and emerging fertility preservation options for women.Journal of gynecologic oncology · 2016Review
- Advances in Isolation Methods for Spermatogonial Stem Cells.Stem cell reviews and reports · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChemotherapy and radiation treatments for cancer and other diseases can cause male infertility. There are currently no options to preserve the fertility of prepubertal boys who are not yet making sperm. Cryopreservation of spermatogonial stem cells (SSCs, obtained via testicular biopsy) followed by autologous transplantation back into the testes at a later date may restore fertility in these patients. However, this approach carries an inherent risk of reintroducing cancer.
methodsTo address this aspect of SSC transplantation safety, prepubertal non-human primate testis cell suspensions were inoculated with MOLT4 T-lymphoblastic leukemia cells and subsequently sorted for cell surface markers CD90 (THY-1) and CD45.
resultsCancer cells segregated to the CD90-/CD45+ fraction and produced tumors in nude mice. Nearly all sorted DEAD box polypeptide 4-positive (VASA+) spermatogonia segregated to the CD90+/CD45- fraction. In a preliminary experiment, a purity check of the sorted putative stem cell fraction (CD90+/CD45-) revealed 0.1% contamination with cancer cells, which was sufficient to produce tumors in nude mice. We hypothesized that the contamination resulted from mis-sorting due to cell clumping and employed singlet discrimination (SD) in four subsequent experiments. Purity checks revealed no cancer cell contamination in the CD90+/CD45- fraction from three of the four SD replicates and these fractions produced no tumors when transplanted into nude mouse testes.
conclusionsWe conclude that spermatogonia can be separated from contaminating malignant cells by fluorescence-activated cell sorting prior to SSC transplantation and that post-sorting purity checks are required to confirm elimination of malignant cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.