ArticleThe Journal of biological chemistry2011
Picomolar nitric oxide signals from central neurons recorded using ultrasensitive detector cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- A Theoretical Analysis of the Effects That the Glycocalyx and the Internal Elastic Lamina Have on Nitric Oxide Concentration Gradients in the Arterial Wall.Antioxidants (Basel, Switzerland) · 2025Article
- Modeling nitric oxide diffusion and plasticity modulation in cerebellar learning.APL bioengineering · 2025Article
- Nitrergic modulation of ion channel function in regulating neuronal excitability.Channels (Austin, Tex.) · 2021Article
- Differential ligand-selective control of opposing enzymatic activities within a bifunctional c-di-GMP enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spatial and temporal patterns of nitric oxide diffusion and degradation drive emergent cerebrovascular dynamics.PLoS computational biology · 2020Article
- Physiological activation and deactivation of soluble guanylate cyclase.Nitric oxide : biology and chemistry · 2018Review
- Structure and Activation of Soluble Guanylyl Cyclase, the Nitric Oxide Sensor.Antioxidants & redox signaling · 2017Review
- Localised intracellular signalling in neurons.The Journal of physiology · 2016Article
- From synaptically localized to volume transmission by nitric oxide.The Journal of physiology · 2016Review
- A review of flux considerations for in vivo neurochemical measurements.The Analyst · 2015Review
- Characterisation and comparison of temporal release profiles of nitric oxide generating donors.Journal of neuroscience methods · 2015Article
- Article
- Redox and nitric oxide-mediated regulation of sensory neuron ion channel function.Antioxidants & redox signaling · 2015Review
- H2S and NO cooperatively regulate vascular tone by activating a neuroendocrine HNO-TRPA1-CGRP signalling pathway.Nature communications · 2014Article
- The role of nitric oxide in pre-synaptic plasticity and homeostasis.Frontiers in cellular neuroscience · 2013Review
- Differences in oxygen-dependent nitric oxide metabolism by cytoglobin and myoglobin account for their differing functional roles.The FEBS journal · 2013Article
- On the selectivity of neuronal NOS inhibitors.British journal of pharmacology · 2013Article
- Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Improved genetically-encoded, FlincG-type fluorescent biosensors for neural cGMP imaging.Frontiers in molecular neuroscience · 2013Article
- Cellular targets of nitric oxide in the hippocampus.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Nitric oxide (NO) is a widespread signaling molecule with potentially multifarious actions of relevance to health and disease. A fundamental determinant of how it acts is its concentration, but there remains a lack of coherent information on the patterns of NO release from its sources, such as neurons or endothelial cells, in either normal or pathological conditions. We have used detector cells having the highest recorded NO sensitivity to monitor NO release from brain tissue quantitatively and in real time. Stimulation of NMDA receptors, which are coupled to activation of neuronal NO synthase, routinely generated NO signals from neurons in cerebellar slices. The average computed peak NO concentrations varied across the anatomical layers of the cerebellum, from 12 to 130 pm. The mean value found in the hippocampus was 200 pm. Much variation in the amplitudes recorded by individual detector cells was observed, this being attributable to their location at variable distances from the NO sources. From fits to the data, the NO concentrations at the source surfaces were 120 pm to 1.4 nm, and the underlying rates of NO generation were 36-350 nm/s, depending on area. Our measurements are 4-5 orders of magnitude lower than reported by some electrode recordings in cerebellum or hippocampus. In return, they establish coherence between the NO concentrations able to elicit physiological responses in target cells through guanylyl cyclase-linked NO receptors, the concentrations that neuronal NO synthase is predicted to generate locally, and the concentrations that neurons actually produce.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.