ArticleNature protocols2011
Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Decoupling Fabrication From Encoding: DNA-Addressable Template Microparticles for Large, User-Defined Optical Barcode Libraries.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- DNA nanotechnology-based strategies for gastric cancer diagnosis and therapy.Materials today. Bio · 2025Review
- Rapid and on-site wireless immunoassay of respiratory virus aerosols via hydrogel-modulated resonators.Nature communications · 2024Article
- Microfluidic Immuno-Serolomic Assay Reveals Systems Level Association with COVID-19 Pathology and Vaccine Protection.Small methods · 2023Article
- CRISPR-Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Shrinkable Hydrogel-Enhanced Biomarker Detection with X-ray Fluorescent Nanoparticles.Nanomaterials (Basel, Switzerland) · 2022Article
- Stop-Flow Lithography for the Continuous Production of Degradable Hydrogel Achiral Crescent Microswimmers.Micromachines · 2022Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Janus particles and motors: unrivaled devices for mastering (bio)sensing.Mikrochimica acta · 2021Review
- Multispectral upconversion nanoparticles for near infrared encoding of wearable devices.RSC advances · 2021Article
- Uncovering the Depths of the Human Proteome: Antibody-based Technologies for Ultrasensitive Multiplexed Protein Detection and Quantification.Molecular & cellular proteomics : MCP · 2021Review
- Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments.Advanced functional materials · 2020Article
- Article
- Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography.Journal of clinical medicine · 2020Article
- Article
- Separation-encoded microparticles for single-cell western blotting.Lab on a chip · 2020Article
- Engineering functional hydrogel microparticle interfaces by controlled oxygen-inhibited photopolymerization.Colloids and surfaces. B, Biointerfaces · 2019Article
- Fabrication of Functional Biomaterial Microstructures by in Situ Photopolymerization and Photodegradation.ACS biomaterials science & engineering · 2018Article
- Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays.Lab on a chip · 2018Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
This protocol describes the core methodology for the fabrication of bar-coded hydrogel microparticles, the capture and labeling of protein targets and the rapid microfluidic scanning of particles for multiplexed detection. Multifunctional hydrogel particles made from poly(ethylene glycol) serve as a sensitive, nonfouling and bio-inert suspension array for the multiplexed measurement of proteins. Each particle type bears a distinctive graphical code consisting of unpolymerized holes in the wafer structure of the microparticle; this code serves to identify the antibody probe covalently incorporated throughout a separate probe region of the particle. The protocol for protein detection can be separated into three steps: (i) synthesis of particles via microfluidic flow lithography at a rate of 16,000 particles per hour; (ii) a 3-4-h assay in which protein targets are captured and labeled within particles using an antibody sandwich technique; and (iii) a flow scanning procedure to detect bar codes and quantify corresponding targets at rates of 25 particles per s. By using the techniques described, single- or multiple-probe particles can be reproducibly synthesized and used in customizable multiplexed panels to measure protein targets over a three-log range and at concentrations as low as 1 pg ml(-1).
Indexed as
Identifiers
22015846What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.