ArticleBiochimica et biophysica acta2012
Cytoskeleton disruption in J774 macrophages: consequences for lipid droplet formation and cholesterol flux.
Article in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- The Role of Neutral Sphingomyelinase-2 (NSM2) in the Control of Neutral Lipid Storage in T Cells.International journal of molecular sciences · 2024Article
- A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK signalling and aberrant lipid homeostasis.iScience · 2023Article
- Lipid Droplet Protein PLIN1 Regulates Inflammatory Polarity in Human Macrophages and is Involved in Atherosclerotic Plaque Development by Promoting Stable Lipid Storage.Journal of atherosclerosis and thrombosis · 2023Article
- ARP2/3 Regulates Fatty Acid Synthesis by Modulating Lipid Droplets' Motility.International journal of molecular sciences · 2022Article
- Touch and Go: Membrane Contact Sites Between Lipid Droplets and Other Organelles.Frontiers in cell and developmental biology · 2022Review
- Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.Autophagy · 2021Article
- The Cell Culture Environment Regulates the Transcription Factor MafB in BV-2 Microglia.Matters · 2021Article
- Progress in the Chemistry of Cytochalasans.Progress in the chemistry of organic natural products · 2021Article
- Effect of Gender to Fat Deposition in Yaks Based on Transcriptomic and Metabolomics Analysis.Frontiers in cell and developmental biology · 2021Article
- Immunomodulation of J774A.1 Murine Macrophages byFrontiers in microbiology · 2020Article
- Transcriptional insights into key genes and pathways controlling muscle lipid metabolism in broiler chickens.BMC genomics · 2019Article
- Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens.BMC genomics · 2018Article
- Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage.Nature communications · 2017Article
- Rab proteins implicated in lipid storage and mobilization.Journal of biomedical research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Macrophages store excess unesterified cholesterol (free, FC) in the form of cholesteryl ester (CE) in cytoplasmic lipid droplets. The hydrolysis of droplet-CE in peripheral foam cells is critical to HDL-promoted reverse cholesterol transport because it represents the first step in cellular cholesterol clearance, as only FC is effluxed from cells to HDL. Cytoplasmic lipid droplets move within the cell utilizing the cytoskeletal network, but, little is known about the influence of the cytoskeleton on lipid droplet formation. To understand this role we employed cytochalasin D (cyt.D) to promote actin depolymerization in J774 macrophages. Incubating J774 with acetylated LDL creates foam cells having a 4-fold increase in cellular cholesterol content (30-40% cholesterol present as cholesteryl ester (CE)) in cytoplasmic droplets. Lipid droplets formed in the presence of cyt.D are smaller in diameter. CE-deposition and -hydrolysis are decreased when cells are cholesterol-enriched in the presence of cyt.D or latrunculin A, another cytoskeleton disrupting agent. However, when lipid droplets formed in the presence of cyt.D are isolated and incubated with an exogenous CE hydrolase, the CE is more rapidly metabolized compared to droplets from control cells. This is apparently due to the smaller size and altered lipid composition of the droplets formed in the presence of cyt.D. Cytoskeletal proteins found on CE droplets influence droplet lipid composition and maturation in model foam cells. In J774 macrophages, cytoskeletal proteins are apparently involved in facilitating the interaction of lipid droplets and a cytosolic neutral CE hydrolase and may play a role in foam cell formation. This article is part of a Special Issue entitled Advances in High Density Lipoprotein Formation and Metabolism: A Tribute to John F. Oram (1945-2010).
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