ArticleMolecular immunology2011
Cofactor regulation of C5a chemotactic activity in physiological fluids. Requirement for the vitamin D binding protein, thrombospondin-1 and its receptors.
Article in Molecular immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF.International journal of molecular sciences · 2023Article
- Transcriptomic analysis reveal the responses of dendritic cells to VDBP.Genes & genomics · 2022Article
- Investigation of brain damage mechanism in middle cerebral artery occlusion/reperfusion rats based on i-TRAQ quantitative proteomics.Experimental brain research · 2021Article
- Article
- Neutrophils in the initiation and resolution of acute pulmonary inflammation: understanding biological function and therapeutic potential.The Journal of pathology · 2019Review
- Loss of acid ceramidase in myeloid cells suppresses intestinal neutrophil recruitment.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Systemic alterations in plasma proteins from women with chronic widespread pain compared to healthy controls: a proteomic study.Journal of pain research · 2017Article
- The role of C5a in acute lung injury induced by highly pathogenic viral infections.Emerging microbes & infections · 2015Review
- MicroRNA and mRNA expression profiling in rat acute respiratory distress syndrome.BMC medical genomics · 2014Article
- Circulating complexes of the vitamin D binding protein with G-actin induce lung inflammation by targeting endothelial cells.Immunobiology · 2014Article
- Neutrophil recruitment to the lung in both C5a- and CXCL1-induced alveolitis is impaired in vitamin D-binding protein-deficient mice.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Factors in physiological fluids that regulate the chemotactic activity of complement activation peptides C5a and C5a des Arg are not well understood. The vitamin D binding protein (DBP) has been shown to significantly enhance chemotaxis to C5a/C5a des Arg. More recently, platelet-derived thrombospondin-1 (TSP-1) has been shown to facilitate the augmentation of C5a-induced chemotaxis by DBP. The objective of this study was to better characterize these chemotactic cofactors and investigate the role that cell surface TSP-1 receptors CD36 and CD47 may play in this process. The chemotactic activity in C-activated normal serum, citrated plasma, DBP-depleted serum or C5 depleted serum was determined for both normal human neutrophils and U937 cell line transfected with the C5a receptor (U937-C5aR). In addition, levels of C5a des Arg, DBP and TSP-1 in these fluids were measured by RIA or ELISA. Results show that there is a clear hierarchy with C5a being the essential primary signal (DBP or TSP-1 will not function in the absence of C5a), DBP the necessary cofactor and TSP-1 a dependent tertiary factor, since it cannot function to enhance chemotaxis to C5a without DBP. Measurement of the C5a-induced intracellular calcium flux confirmed the same hierarchy observed with chemotaxis. Moreover, analysis of bronchoalveolar lavage fluid (BALF) from patients with the adult respiratory distress syndrome (ARDS) demonstrated that C5a-dependent chemotactic activity is significantly decreased after anti-DBP treatment. Finally, results show that TSP-1 utilizes cell surface receptors CD36 and CD47 to augment chemotaxis, but DBP does not bind to TSP-1, CD36 or CD47. The results clearly demonstrate that C5a/C5a des Arg needs both DBP and TSP-1 for maximal chemotactic activity and suggest that the regulation of C5a chemotactic activity in physiological fluids is more complex than previously thought.
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