ArticleJournal of comparative pathology2012
Uropathogenic E. coli promote a paracellular urothelial barrier defect characterized by altered tight junction integrity, epithelial cell sloughing and cytokine release.
Article in Journal of comparative pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Human bladder organoids model urinary tract infection and bacteriophage therapy.bioRxiv : the preprint server for biology · 2025Article
- Article
- Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model.Scientific reports · 2024Article
- Cranberry Polyphenols and Prevention against Urinary Tract Infections: New Findings Related to the Integrity and Functionality of Intestinal and Urinary Barriers.Journal of agricultural and food chemistry · 2024Article
- Insulin receptor signaling engages bladder urothelial defenses that limit urinary tract infection.Cell reports · 2024Article
- Unraveling the complexity of bladder-centric chronic pain by intravesical contrast enhanced MRI.Continence (Amsterdam, Netherlands) · 2023Article
- Effective Treatments of UTI-Is Intravesical Therapy the Future?Pathogens (Basel, Switzerland) · 2023Review
- Cranberry, but not D-mannose and ibuprofen, prevents against uropathogenicFrontiers in microbiology · 2023Article
- A stable cyclized antimicrobial peptide derived from LL-37 with host immunomodulatory effects and activity against uropathogens.Cellular and molecular life sciences : CMLS · 2022Article
- Urinary Tract Infection in Overactive Bladder: An Update on Pathophysiological Mechanisms.Frontiers in physiology · 2022Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Vitamin D strengthens the bladder epithelial barrier by inducing tight junction proteins during E. coli urinary tract infection.Cell and tissue research · 2020Article
- Review
- Host and Bacterial Markers that Differ in Children with Cystitis and Pyelonephritis.The Journal of pediatrics · 2019Article
- Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro.FEMS microbiology letters · 2018Article
- Role of neurogenic inflammation in local communication in the visceral mucosa.Seminars in immunopathology · 2018Review
- Urine: Waste product or biologically active tissue?Neurourology and urodynamics · 2018Article
- Mechanisms Underlying Overactive Bladder and Interstitial Cystitis/Painful Bladder Syndrome.Frontiers in neuroscience · 2018Review
- High molecular weight hyaluronic acid: a two-pronged protectant against infection of the urogenital tract?Clinical & translational immunology · 2018Article
- Role of renal urothelium in the development and progression of kidney disease.Pediatric nephrology (Berlin, Germany) · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The urinary bladder is a common site of bacterial infection with a majority of cases attributed to uropathogenic Escherichia coli. Sequelae of urinary tract infections (UTIs) include the loss of urothelial barrier function and subsequent clinical morbidity secondary to the permeation of urine potassium, urea and ammonia into the subepithelium. To date there has been limited research describing the mechanism by which this urothelial permeability defect develops. The present study models acute uropathogenic E. coli infection in vitro using intact canine bladder mucosa mounted in Ussing chambers to determine whether infection induces primarily a transcellular or paracellular permeability defect. The Ussing chamber sustains tissue viability while physically separating submucosal and lumen influences, so this model is ideal for quantitative measurement of transepithelial electrical resistance (TER) to assess alterations of urothelial barrier function. Using this model, changes in both tissue ultrastructure and TER indicated that uropathogenic E. coli infection promotes a paracellular permeability defect associated with the failure of umbrella cell tight junction formation and umbrella cell sloughing. In addition, bacterial interaction with the urothelium promoted secretion of cytokines from the urinary bladder with bioactivity capable of modulating epithelial barrier function including tumour necrosis factor-α, interleukin (IL)-6 and IL-15. IL-15 secretion by the infected bladder mucosa is a novel finding and, because IL-15 plays key roles in reconstitution of tight junction function in damaged intestine, this study points to a potential role for IL-15 in UTI-induced urothelial injury.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.