ArticleDiseases of aquatic organisms2011
Development of a sensitive assay for the detection of Pseudoloma neurophilia in laboratory populations of the zebrafish Danio rerio.
Article in Diseases of aquatic organisms, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Application of an eDNA assay for the detection ofAquaculture (Amsterdam, Netherlands) · 2023Article
- MULTI-STATE OCCUPANCY MODEL ESTIMATES PROBABILITY OF DETECTION OF AN AQUATIC PARASITE USING ENVIRONMENTAL DNA: PSEUDOLOMA NEUROPHILIA IN ZEBRAFISH AQUARIA.The Journal of parasitology · 2022Article
- The Effects of Water Volume and Bacterial Concentration on the Water Filtration Assay Used in Zebrafish Health Surveillance.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Review of diseases and health management in zebrafish Danio rerio (Hamilton 1822) in research facilities.Journal of fish diseases · 2020Review
- Behavioural effects of the common brain-infecting parasite Pseudoloma neurophilia in laboratory zebrafish (Danio rerio).Scientific reports · 2020Article
- Article
- Comparison of Antemortem and Environmental Samples for Zebrafish Health Monitoring and Quarantine.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Controlling Endemic Pathogens-Challenges and Opportunities.Zebrafish · 2016Article
- Article
- Detection of Nosema bombycis by FTA cards and loop-mediated isothermal amplification (LAMP).Current microbiology · 2014Article
- Unusual fluorescent granulomas and myonecrosis in Danio rerio infected by the microsporidian pathogen Pseudoloma neurophilia.Zebrafish · 2014Article
- Verification of intraovum transmission of a microsporidium of vertebrates: Pseudoloma neurophilia infecting the Zebrafish, Danio rerio.PloS one · 2013Article
- Microsporidiosis in zebrafish research facilities.ILAR journal · 2012Review
- Transmission, diagnosis, and recommendations for control of Pseudoloma neurophilia infections in laboratory zebrafish (Danio rerio) facilities.Comparative medicine · 2011Review
- Development and maintenance of a specific pathogen-free (SPF) zebrafish research facility for Pseudoloma neurophilia.Diseases of aquatic organisms · 2011Article
- Early development and tissue distribution of Pseudoloma neurophilia in the zebrafish, Danio rerio.The Journal of eukaryotic microbiologyArticle
- Review of Pseudoloma neurophilia (Microsporidia): A common neural parasite of laboratory zebrafish (Danio rerio).The Journal of eukaryotic microbiologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The zebrafish Danio rerio is an increasingly important biological model in many areas of research. Due to the potential for non-protocol-induced variation, diseases of zebrafish, especially those resulting in chronic, sub-lethal infections, are of great concern. The microsporidium Pseudoloma neurophilia is a common parasite of laboratory zebrafish. Current methods for detection of this parasite require lethal sampling of fish, which is often undesirable with poorly spawning mutant lines and small populations. We present here an improved molecular-based diagnostic assay using real-time polymerase chain reaction (PCR), and including sonication treatment prior to DNA extraction. Comparisons of several DNA extraction methods were performed to determine the method providing the maximum sensitivity. Sonication was found to be the most effective method for disrupting spores. Compared to previously published data on PCR-based assay using a dilution experiment, sensitivity is increased. This shows that our assay, which includes sonication, is capable of detecting parasite DNA at 1 log higher dilution than the conventional PCR-based assay, which does not include sonication. Furthermore, we demonstrate the application of this method to testing of water, eggs, and sperm, providing a potential non-lethal method for detection of this parasite in zebrafish colonies with a sensitivity of 10 spores 1(-1) of water, 2 spores per spiked egg sample, and 10 spores microl(-1) of spiked sperm sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.