ArticleJournal of biomedicine & biotechnology2011
Cloning and characterization of the ζ-carotene desaturase gene from Chlorella protothecoides CS-41.
Article in Journal of biomedicine & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Harnessing systems biology approach for characterization of carotenoid biosynthesis pathways in microalgae.Biochemistry and biophysics reports · 2024Review
- Genome-based identification and comparative analysis of enzymes for carotenoid biosynthesis in microalgae.World journal of microbiology & biotechnology · 2021Article
- Whole genome sequencing ofPeerJ · 2017Article
- Isolation and Analysis of the Cppsy Gene and Promoter from Chlorella protothecoides CS-41.Marine drugs · 2015Article
- Hypoosmotic expression of Dunaliella bardawil ζ-carotene desaturase is attributed to a hypoosmolarity-responsive element different from other key carotenogenic genes.Plant physiology · 2014Article
- Structure and function characterization of the phytoene desaturase related to the lutein biosynthesis in Chlorella protothecoides CS-41.Molecular biology reports · 2013Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
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Abstract
To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the ζ-carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively. Phylogenetic analysis demonstrated that the ZDS from C. protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species. In addition, we also found that the zds gene expression was upregulated in response to light.
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