ArticleJournal of virology2012
The oral secretion of infectious scrapie prions occurs in preclinical sheep with a range of PRNP genotypes.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- BSE can propagate in sheep co-infected or pre-infected with scrapie.Scientific reports · 2021Article
- Classical scrapie in small ruminants is caused by at least four different prion strains.Veterinary research · 2021Article
- Classical and Atypical Scrapie in Sheep and Goats. Review on the Etiology, Genetic Factors, Pathogenesis, Diagnosis, and Control Measures of Both Diseases.Animals : an open access journal from MDPI · 2021Review
- Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk.Prion · 2020Article
- Neuroimmune Response in Natural Preclinical Scrapie after Dexamethasone Treatment.International journal of molecular sciences · 2020Article
- Evolutionary biology and the risk of scrapie disease in sheep.Open veterinary journal · 2018Review
- How do PrPPathogens (Basel, Switzerland) · 2017Review
- Prenatal transmission of scrapie in sheep and goats: A case study for veterinary public health.Open veterinary journal · 2016Review
- Longitudinal Detection of Prion Shedding in Saliva and Urine by Chronic Wasting Disease-Infected Deer by Real-Time Quaking-Induced Conversion.Journal of virology · 2015Article
- Circulation of prions within dust on a scrapie affected farm.Veterinary research · 2015Article
- Mitigation of prion infectivity and conversion capacity by a simulated natural process--repeated cycles of drying and wetting.PLoS pathogens · 2015Article
- Objects in Contact with Classical Scrapie Sheep Act as a Reservoir for Scrapie Transmission.Frontiers in veterinary science · 2015Article
- Rapid antemortem detection of CWD prions in deer saliva.PloS one · 2013Article
- Small-molecule theranostic probes: a promising future in neurodegenerative diseases.International journal of cell biology · 2013Review
- Prion in saliva of bovine spongiform encephalopathy-infected cattle.Emerging infectious diseases · 2012Article
- In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation.BMC veterinary research · 2012Article
- Sensitivity of protein misfolding cyclic amplification versus immunohistochemistry in ante-mortem detection of chronic wasting disease.The Journal of general virology · 2012Article
- Animal prion diseases: A review of intraspecies transmission.Open veterinary journalReview
- Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preclinical sheep with the highly scrapie-susceptible VRQ/VRQ PRNP genotype secrete prions from the oral cavity. In order to further understand the significance of orally available prions, buccal swabs were taken from sheep with a range of PRNP genotypes and analyzed by serial protein misfolding cyclic amplification (sPMCA). Prions were detected in buccal swabs from scrapie-exposed sheep of genotypes linked to high (VRQ/VRQ and ARQ/VRQ) and low (ARR/VRQ and AHQ/VRQ) lymphoreticular system involvement in scrapie pathogenesis. For both groups, the level of prion detection was significantly higher than that for scrapie-resistant ARR/ARR sheep which were kept in the same farm environment and acted as sentinel controls for prions derived from the environment which might contaminate the oral cavity. In addition, sheep with no exposure to the scrapie agent did not contain any measurable prions within the oral cavity. Furthermore, prions were detected in sheep over a wide age range representing various stages of preclinical disease. These data demonstrate that orally available scrapie prions may be a common feature in sheep incubating scrapie, regardless of the PRNP genotype and any associated high-level accumulation of PrP(Sc) within lymphoreticular tissues. PrP(Sc) was present in buccal swabs from a large proportion of sheep with PRNP genotypes associated with relatively low disease penetrance, indicating that subclinical scrapie infection is likely to be a common occurrence. The significance of positive sPMCA reactions was confirmed by the transmission of infectivity in buccal swab extracts to Tg338 mice, illustrating the likely importance of orally available prions in the horizontal transmission of scrapie.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.