ArticleBiological procedures online2011
An automated cell-counting algorithm for fluorescently-stained cells in migration assays.
Article in Biological procedures online, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition.NPJ precision oncology · 2023Article
- Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models.Journal of tissue engineering and regenerative medicine · 2023Article
- Automated cell counting for Trypan blue-stained cell cultures using machine learning.PloS one · 2023Article
- Semi-automated quantification of hair cells in the mature mouse utricle.Hearing research · 2022Article
- Micro-endoscopy for Live Small Animal Fluorescent Imaging.Advances in experimental medicine and biology · 2021Article
- Deep learning-based light scattering microfluidic cytometry for label-free acute lymphocytic leukemia classification.Biomedical optics express · 2020Article
- Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay.Biological procedures online · 2017Article
- Automated analysis of co-localized protein expression in histologic sections of prostate cancer.PloS one · 2017Article
- Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins.Journal of visualized experiments : JoVE · 2016Article
- Automatic stage identification of Drosophila egg chamber based on DAPI images.Scientific reports · 2016Article
- Differential expression of doublecortin and microglial markers in the rat brain following fractionated irradiation.Neurochemical research · 2015Article
- CELLCOUNTER: novel open-source software for counting cell migration and invasion in vitro.BioMed research international · 2014Article
- Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images.Biological procedures online · 2013Article
- Using Bland-Altman Analysis to Identify Appropriate Clonogenic Assay Colony Counting Techniques.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cell-counting algorithm, developed in Matlab®, was created to efficiently count migrated fluorescently-stained cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images were acquired at 2.5 ×, 5 ×, and 10 × objective magnifications. Automated cell counts strongly correlated to manual counts (r2 = 0.99, P < 0.0001 for a total of 47 images), with no difference in the measurements between methods under all conditions. We conclude that our automated method is accurate, more efficient, and void of variability and potential observer bias normally associated with manual counting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.