ArticlePhotochemistry and photobiology
Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET.
Article in Photochemistry and photobiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.Science advances · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.bioRxiv : the preprint server for biology · 2024Article
- Genetically Encoded Fluorescent Probe for Detection of Heme-Induced Conformational Changes in Cytochrome c.Biosensors · 2023Article
- Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway.ACS physical chemistry Au · 2023Review
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Imaging Voltage with Microbial Rhodopsins.Frontiers in molecular biosciences · 2021Review
- Advanced Near-Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Sub-20 nm Core-Shell-Shell Nanoparticles for Bright Upconversion and Enhanced Förster Resonant Energy Transfer.Journal of the American Chemical Society · 2019Article
- Genetic voltage indicators.BMC biology · 2019Review
- Optogenetics: Background, Methodological Advances and Potential Applications for Cardiovascular Research and Medicine.Frontiers in bioengineering and biotechnology · 2019Review
- Raman spectroscopy of a near infrared absorbing proteorhodopsin: Similarities to the bacteriorhodopsin O photointermediate.PloS one · 2018Article
- Live-cell imaging of cell signaling using genetically encoded fluorescent reporters.The FEBS journal · 2018Review
- Voltage Imaging: Pitfalls and Potential.Biochemistry · 2017Review
- Structural Changes in an Anion Channelrhodopsin: Formation of the K and L Intermediates at 80 K.Biochemistry · 2017Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Genetically encoded indicators of neuronal activity.Nature neuroscience · 2016Review
- The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.Current opinion in chemical biology · 2015Review
- Using total internal reflection fluorescence microscopy to visualize rhodopsin-containing cells.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Microbial rhodopsins are an important class of light-activated transmembrane proteins whose function is typically studied on bulk samples. Herein, we apply photochromic fluorescence resonance energy transfer to investigate the dynamics of these proteins with sensitivity approaching the single-molecule limit. The brightness of a covalently linked organic fluorophore is modulated by changes in the absorption spectrum of the endogenous retinal chromophore that occur as the molecule undergoes a light-activated photocycle. We studied the photocycles of blue-absorbing proteorhodopsin and sensory rhodopsin II (SRII). Clusters of 2-3 molecules of SRII clearly showed a light-induced photocycle. Single molecules of SRII showed a photocycle upon signal averaging over several illumination cycles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.