ArticleTissue engineering. Part C, Methods2012
Nondestructive evaluation of tissue engineered articular cartilage using time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Assessing the micromechanical architecture of joint tissues with speckle rheological microscopy.Nature communications · 2026Article
- On the Nature of Stationary and Time-Resolved Fluorescence Spectroscopy of Collagen Powder from Bovine Achilles Tendon.International journal of molecular sciences · 2023Article
- Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model.Journal of biomedical optics · 2023Article
- Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.Scientific reports · 2020Article
- Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine.Tissue engineering. Part B, Reviews · 2020Review
- Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging.Journal of biomedical optics · 2018Article
- Nondestructive/Noninvasive Imaging Evaluation of Cellular Differentiation Progression During In Vitro Mesenchymal Stem Cell-Derived Chondrogenesis.Tissue engineering. Part A · 2018Article
- Noninvasive Optical Assessment of Implanted Engineered Tissues Correlates with Cytokine Secretion.Tissue engineering. Part C, Methods · 2018Article
- Detection of Pentosidine Cross-Links in Cell-Secreted Decellularized Matrices Using Time Resolved Fluorescence Spectroscopy.ACS biomaterials science & engineering · 2017Article
- Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage.Annals of biomedical engineering · 2016Review
- Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials.Annals of biomedical engineering · 2016Review
- Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review.Annals of biomedical engineering · 2016Review
- Generation and Assessment of Functional Biomaterial Scaffolds for Applications in Cardiovascular Tissue Engineering and Regenerative Medicine.Advanced healthcare materials · 2015Review
- Identification of cartilage injury using quantitative multiphoton microscopy.Osteoarthritis and cartilage · 2014Article
- Imaging challenges in biomaterials and tissue engineering.Biomaterials · 2013Article
- Spatiotemporal Characterization of Extracellular Matrix Microstructures in Engineered Tissue: A Whole-Field Spectroscopic Imaging Approach.Journal of nanotechnology in engineering and medicine · 2013Article
- Induced collagen cross-links enhance cartilage integration.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The goal of this study is to evaluate the ability of a bimodal technique integrating time-resolved fluorescence spectroscopy (TRFS) and ultrasound backscatter microscopy (UBM) for nondestructive detection of changes in the biochemical, structural, and mechanical properties of self-assembled engineered articular cartilage constructs. The cartilage constructs were treated with three chemical agents (collagenase, chondroitinase-ABC, and ribose) to induce changes in biochemical content (collagen and glycosaminoglycan [GAG]) of matured constructs (4 weeks); and to subsequently alter the mechanical properties of the construct. The biochemical changes were evaluated using TRFS. The microstructure and the thickness of the engineered cartilage samples were characterized by UBM. The optical and ultrasound results were validated against those acquired via conventional techniques including collagen and GAG quantification and measurement of construct stiffness. Current results demonstrated that a set of optical parameters (e.g., average fluorescence lifetime and decay constants) showed significant correlation (p<0.05) with biochemical and mechanical data. The high-resolution ultrasound images provided complementary cross-section information of the cartilage samples morphology. Therefore, the technique was capable of nondestructively evaluating the composition of extracellular matrix and the microstructure of engineered tissue, demonstrating great potential as an alternative to traditional destructive assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.