ArticleProceedings of the National Academy of Sciences of the United States of America1990
cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 84 citations in OpenAlex.
- Interactions ofMicrobiology spectrum · 2026Article
- A Novel TLR4-Binding Domain of Peroxiredoxin FromFrontiers in immunology · 2021Article
- The cell surface proteome of Entamoeba histolytica.Molecular & cellular proteomics : MCP · 2014Article
- Differential expression and immunolocalization of antioxidant enzymes in Entamoeba histolytica isolates during metronidazole stress.BioMed research international · 2014Article
- Peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess.BioMed research international · 2014Review
- Structural and biochemical characterization of peroxiredoxin Qbeta from Xylella fastidiosa: catalytic mechanism and high reactivity.The Journal of biological chemistry · 2010Article
- Prediction of carbohydrate-binding proteins from sequences using support vector machines.Advances in bioinformatics · 2010Article
- Schistosome infection stimulates host CD4(+) T helper cell and B-cell responses against a novel egg antigen, thioredoxin peroxidase.Infection and immunity · 2001Article
- Human and murine immune responses to a novel Leishmania major recombinant protein encoded by members of a multicopy gene family.Infection and immunity · 1998Article
- Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica.The Biochemical journal · 1998Article
- Progress towards development of a vaccine for amebiasis.Clinical microbiology reviews · 1997Review
- Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.The Biochemical journal · 1997Article
- Kinetoplastid glucose transporters.The Biochemical journal · 1997Review
- Primary structure and expression of plant homologues of animal and fungal thioredoxin-dependent peroxide reductases and bacterial alkyl hydroperoxide reductases.Plant molecular biology · 1996Article
- Cloning and expression of a putative alcohol dehydrogenase gene of Entamoeba histolytica and its application to immunological examination.Clinical and diagnostic laboratory immunology · 1996Article
- pag gene-like protein (ABP-25) of the Cynops embryo: regional distribution and gene expression during early embryogenesis.Roux's archives of developmental biology : the official organ of the EDBO · 1995Article
- Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli.The Biochemical journal · 1995Article
- Molecular cloning of a 30-kilodalton lysine-rich surface antigen from a nonpathogenic Entamoeba histolytica strain and its expression in a pathogenic strain.Infection and immunity · 1995Article
- Protection of gerbils from amebic liver abscess by immunization with recombinant Entamoeba histolytica 29-kilodalton antigen.Infection and immunity · 1995Article
- Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A gamma gt11 cDNA library was constructed from poly(U)-Sepharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit polyclonal anti-E. histolytica serum. A 700-base-pair cDNA insert was isolated and the nucleotide sequence was determined. The deduced amino acid sequence of the cDNA revealed a cysteine-rich protein. DNA hybridizations showed that the gene was specific to E. histolytica since the cDNA probe reacted with DNA from four axenic strains of E. histolytica but did not react with DNA from Entamoeba invadens, Acanthamoeba castellanii, or Trichomonas vaginalis. The insert was subcloned into the expression vector pGEX-1 and the protein was expressed as a fusion with the C terminus of glutathione S-transferase. Purified fusion protein was used to generate 22 monoclonal antibodies (mAbs) and a mouse polyclonal antiserum specific for the E. histolytica portion of the fusion protein. A 29-kDa protein was identified as a surface antigen when mAbs were used to immunoprecipitate the antigen from metabolically 35S-labeled live trophozoites. The surface location of the antigen was corroborated by mAb immunoprecipitation of a 29-kDa protein from surface-125I-labeled whole trophozoites as well as by the reaction of mAbs with live trophozoites in an indirect immunofluorescence assay performed at 4 degrees C. Immunoblotting with mAbs demonstrated that the antigen was present on four axenic isolates tested. mAbs recognized epitopes on the 29-kDa native antigen on some but not all clinical isolates tested.
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