ArticleThe Plant cell2011
Single-molecule analysis of PIP2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed, 267 citations in OpenAlex.
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- SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants.Nature communications · 2025Article
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- How sterols affect protoplasts plasma membrane water permeability and their volume under osmotic shock.Journal of plant research · 2025Article
- eIF2Bβ confers resistance to Turnip mosaic virus by recruiting ALKBH9B to modify viral RNA methylation.Plant biotechnology journal · 2024Article
- The MsDHN1-MsPIP2;1-MsmMYB module orchestrates the trade-off between growth and survival of alfalfa in response to drought stress.Plant biotechnology journal · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip.Nature communications · 2024Article
- AtPIP1;4 and AtPIP2;4 Cooperatively Mediate HPlants (Basel, Switzerland) · 2024Article
- Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate.Nature communications · 2023Article
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.Nature communications · 2023Article
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.bioRxiv : the preprint server for biology · 2023Article
- Involvement of the Membrane Nanodomain Protein,International journal of molecular sciences · 2023Article
- Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress.Plant physiology · 2023Article
- Plant elicitor peptide induces endocytosis of plasma membrane proteins in Arabidopsis.Frontiers in plant science · 2023Article
- Exploring aquaporin functions during changes in leaf water potential.Frontiers in plant science · 2023Review
52 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PIP2;1 is an integral membrane protein that facilitates water transport across plasma membranes. To address the dynamics of Arabidopsis thaliana PIP2;1 at the single-molecule level as well as their role in PIP2;1 regulation, we tracked green fluorescent protein-PIP2;1 molecules by variable-angle evanescent wave microscopy and fluorescence correlation spectroscopy (FCS). Single-particle tracking analysis revealed that PIP2;1 presented four diffusion modes with large dispersion of diffusion coefficients, suggesting that partitioning and dynamics of PIP2;1 are heterogeneous and, more importantly, that PIP2;1 can move into or out of membrane microdomains. In response to salt stress, the diffusion coefficients and percentage of restricted diffusion increased, implying that PIP2;1 internalization was enhanced. This was further supported by the decrease in PIP2;1 density on plasma membranes by FCS. We additionally demonstrated that PIP2;1 internalization involves a combination of two pathways: a tyrphostin A23-sensitive clathrin-dependent pathway and a methyl-β-cyclodextrin-sensitive, membrane raft-associated pathway. The latter was efficiently stimulated under NaCl conditions. Taken together, our findings demonstrate that PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1, suggesting that the dynamic partitioning and recycling pathways might be involved in the multiple modes of regulating water permeability.
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