ArticleOrthopaedic surgery2010
Dynamic expression of Runx2, Osterix and AJ18 in the femoral head of steroid-induced osteonecrosis in rats.
Article in Orthopaedic surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Differential connectivity of gene regulatory networks distinguishes corticosteroid response in asthma.The Journal of allergy and clinical immunology · 2018Trial
- Pigeonpea leaves in orthopedic diseases: bioactive metabolites, pharmacological actions and therapeutic potential.Frontiers in pharmacology · 2026Review
- SOST/Sclerostin impairs the osteogenesis and angiogesis in glucocorticoid-associated osteonecrosis of femoral head.Molecular medicine (Cambridge, Mass.) · 2024Article
- Neohesperidin promotes the osteogenic differentiation of human bone marrow stromal cells by inhibiting the histone modifications of lncRNA SNHG1.Cell cycle (Georgetown, Tex.) · 2021Article
- The Polymorphism at PLCB4 Promoter (rs6086746) Changes the Binding Affinity of RUNX2 and Affects Osteoporosis Susceptibility: An Analysis of Bioinformatics-Based Case-Control Study and Functional Validation.Frontiers in endocrinology · 2021Article
- Impact of the Host-Microbiome on Osteomyelitis Pathogenesis.Frontiers in molecular biosciences · 2021Review
- Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR.Drug design, development and therapy · 2020Article
- Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis.Cell death and differentiation · 2019Article
- Identification of differentially expressed genes in hip cartilage with femoral head necrosis, based on genome‑wide expression profiles.Molecular medicine reports · 2019Article
- LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation.Scientific reports · 2018Article
- Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect.Scientific reports · 2017Article
- Vitamin K2 promotes mesenchymal stem cell differentiation by inhibiting miR‑133a expression.Molecular medicine reports · 2017Article
- miR-27a attenuates adipogenesis and promotes osteogenesis in steroid-induced rat BMSCs by targeting PPARγ and GREM1.Scientific reports · 2016Article
- P-glycoprotein overexpression in bone marrow-derived multipotent stromal cells decreases the risk of steroid-induced osteonecrosis in the femoral head.Journal of cellular and molecular medicine · 2016Article
- Vitamin K2 Prevents Glucocorticoid-induced Osteonecrosis of the Femoral Head in Rats.International journal of biological sciences · 2016Article
- Association of toll-like receptor 4 signaling pathway with steroid-induced femoral head osteonecrosis in rats.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2014Article
- Transcription factor Runx2 in the low bone mineral density of girls with adolescent idiopathic scoliosis.Orthopaedic surgery · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo study dynamic changes in gene expression and protein synthesis of runt-related transcription factor-2 (Runx2), Osterix and AJ18 in the femoral head of steroid-induced osteonecrosis in rats.
methodsForty mature Wistar rats, 250-270 g (mean, 260 g) in weight, were randomly divided into model (30 rats) and control groups (10 rats). An early rat model of femoral head necrosis (FHN) was created by weekly injections of dexamethasone (20 mg/kg) into alternate sides of the gluteus maximus and twice-weekly training on a laboratory animal treadmill for 8 weeks. Hematoxylin and eosin (HE) staining was used to determine whether osteonecrosis had been successfully induced, and the model was then divided into equal 8, 10 and 12 week groups. At the end of the appropriate time period, total RNA and total protein were extracted from the femoral heads, and then real-time quantitative polymerase chain reaction and Western blot were performed to detect dynamic changes in the expression of Runx2, Osterix and AJ18 and protein synthesis in femoral heads with steroid-induced osteonecrosis in rats.
resultsAt the post-modeling eighth, tenth and twelfth week, expression of Runx2 mRNA, Osterix mRNA and related protein synthesis were significantly down-regulated compared to that of the control group, which showed a downward trend with time; while expression of AJ18 mRNA and protein synthesis in the model group was much higher than in the control group, which showed an upward trend with time.
conclusionGlucocorticoids may induce femoral head osteonecrosis by down-regulating Runx2/Osterix mRNA and up-regulating AJ18 mRNA.
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