ArticleNucleic acids research2012
Molecular basis of differential target regulation by miR-96 and miR-182: the Glypican-3 as a model.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Expression study of microRNA cluster on chromosome 19 (C19MC) in tumor tissue and serum of breast cancer patient.Molecular biology reports · 2023Article
- MicroRNAs in the Mouse Developing Retina.International journal of molecular sciences · 2023Review
- MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer.SAGE open medicine · 2023Article
- Inhibition of MicroRNA-182/183 Cluster Ameliorates Schizophrenia by Activating the Axon Guidance Pathway and Upregulating DCC.Oxidative medicine and cellular longevity · 2022Article
- The Role of Glypicans in Cancer Progression and Therapy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Review
- Sensational MicroRNAs: Neurosensory Roles of the MicroRNA-183 Family.Molecular neurobiology · 2020Review
- MiRNA Regulatory Functions in Photoreceptors.Frontiers in cell and developmental biology · 2020Review
- miR-96 promotes invasion and metastasis by targeting GPC3 in non-small cell lung cancer cells.Oncology letters · 2018Article
- ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells.Oncogene · 2018Article
- Article
- The potential of miR-183 family expression in inner ear for regeneration, treatment, diagnosis and prognosis of hearing loss.Journal of otology · 2017Review
- MicroRNA therapy inhibits hepatoblastoma growthHepatology communications · 2017Article
- Selective activation of miRNAs of the primate-specific chromosome 19 miRNA cluster (C19MC) in cancer and stem cells and possible contribution to regulation of apoptosis.Journal of biomedical science · 2017Article
- The microRNA-183 cluster: the family that plays together stays together.Nucleic acids research · 2015Review
- Expression of microRNA-96 and its potential functions by targeting FOXO3 in non-small cell lung cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- MicroRNA-320b promotes colorectal cancer proliferation and invasion by competing with its homologous microRNA-320a.Cancer letters · 2015Article
- Serum miR-96 is a promising biomarker for hepatocellular carcinoma in patients with chronic hepatitis B virus infection.International journal of clinical and experimental medicine · 2015Article
- Roles of miR-182 in sensory organ development and cancer.Thoracic cancer · 2015Review
- miR-92a regulates expression of synaptic GluA1-containing AMPA receptors during homeostatic scaling.Nature neuroscience · 2014Article
- GPC1 regulated by miR-96-5p, rather than miR-182-5p, in inhibition of pancreatic carcinoma cell proliferation.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Besides the fact that miR-96 and miR-182 belong to the miR-182/183 cluster, their seed region (UUGGCA, nucleotides 2-7) is identical suggesting potential common properties in mRNA target recognition and cellular functions. Here, we used the mRNA encoding Glypican-3, a heparan-sulfate proteoglycan, as a model target as its short 3' untranslated region is predicted to contain one miR-96/182 site, and assessed whether it is post-transcriptionally regulated by these two microRNAs. We found that miR-96 downregulated GPC3 expression by targeting its mRNA 3'-untranslated region and interacting with the predicted site. This downregulatory effect was due to an increased mRNA degradation and depended on Argonaute-2. Despite its seed similarity with miR-96, miR-182 was unable to regulate GPC3. This differential regulation was confirmed on two other targets, FOXO1 and FN1. By site-directed mutagenesis, we demonstrated that the miRNA nucleotide 8, immediately downstream the UUGGCA seed, plays a critical role in target recognition by miR-96 and miR-182. Our data suggest that because of a base difference at miRNA position 8, these two microRNAs control a completely different set of genes and therefore are functionally independent.
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