ArticleJournal of biomolecular NMR2011
Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins.
Article in Journal of biomolecular NMR, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Accurate measurement of longitudinal paramagnetic relaxation enhancement through division after subtraction.Magnetic resonance letters · 2027Article
- Enabling Quantitative BenchtopMagnetic resonance in chemistry : MRC · 2026Article
- NMR-based quantification of liquid products in COCommunications chemistry · 2023Article
- Review
- The contribution of electrostatics to hydrogen exchange in the unfolded protein state.Biophysical journal · 2021Article
- AcceleratingACS omega · 2021Article
- Multiple Site-Specific Phosphorylation of IDPs Monitored by NMR.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Protein Structure Determination in Living Cells.International journal of molecular sciences · 2019Review
- Maximizing NMR signal per unit time by facilitating the e-e-n cross effect DNP rate.Physical chemistry chemical physics : PCCP · 2018Article
- MOAG-4 promotes the aggregation of α-synuclein by competing with self-protective electrostatic interactions.The Journal of biological chemistry · 2017Article
- Sensitivity enhancement for membrane proteins reconstituted in parallel and perpendicular oriented bicelles obtained by using repetitive cross-polarization and membrane-incorporated free radicals.Journal of biomolecular NMR · 2017Article
- Applications of NMR spectroscopy to systems biochemistry.Progress in nuclear magnetic resonance spectroscopy · 2016Review
- (19)F Paramagnetic Relaxation Enhancement: A Valuable Tool for Distance Measurements in Proteins.Angewandte Chemie (International ed. in English) · 2016Article
- UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection.Journal of biomolecular NMR · 2016Article
- Paramagnetic relaxation enhancement for protein-observedRSC advances · 2016Article
- HN-NCA heteronuclear TOCSY-NH experiment for (1)H(N) and (15)N sequential correlations in ((13)C, (15)N) labelled intrinsically disordered proteins.Journal of biomolecular NMR · 2015Article
- Increasing the sensitivity of NMR diffusion measurements by paramagnetic longitudinal relaxation enhancement, with application to ribosome-nascent chain complexes.Journal of biomolecular NMR · 2015Article
- Optimized co-solute paramagnetic relaxation enhancement for the rapid NMR analysis of a highly fibrillogenic peptide.Journal of biomolecular NMR · 2015Article
- Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data.Journal of biomolecular NMR · 2015Article
- Measuring translational diffusion coefficients of peptides and proteins by PFG-NMR using band-selective RF pulses.European biophysics journal : EBJ · 2014Article
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5 authors.
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Abstract
We report enhanced sensitivity NMR measurements of intrinsically disordered proteins in the presence of paramagnetic relaxation enhancement (PRE) agents such as Ni(2+)-chelated DO2A. In proton-detected (1)H-(15)N SOFAST-HMQC and carbon-detected (H-flip)(13)CO-(15)N experiments, faster longitudinal relaxation enables the usage of even shorter interscan delays. This results in higher NMR signal intensities per units of experimental time, without adverse line broadening effects. At 40 mmol·L(-1) of the PRE agent, we obtain a 1.7- to 1.9-fold larger signal to noise (S/N) for the respective 2D NMR experiments. High solvent accessibility of intrinsically disordered protein (IDP) residues renders this class of proteins particularly amenable to the outlined approach.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.