ArticleMolecular & cellular proteomics : MCP2012
Integrated transcriptomic and proteomic analysis of the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions of cold and water activity stress.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 86 citations in OpenAlex.
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- Non-canonical transcriptional start sites in E. coli O157:H7 EDL933 are regulated and appear in surprisingly high numbers.BMC microbiology · 2023Article
- Effects of rehydration on physiological and transcriptional responses of a water-stressed rhizobium.Journal of microbiology (Seoul, Korea) · 2022Article
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- Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress.BMC microbiology · 2020Article
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- Unified feature association networks through integration of transcriptomic and proteomic data.PLoS computational biology · 2019Article
- Susceptibility of Escherichia coli O157:H7 grown at low temperatures to the krypton-chlorine excilamp.Scientific reports · 2019Article
- Proteomic and Metabolomic Profiling ofFrontiers in microbiology · 2019Article
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- Overexpressed L20 Rescues 50S Ribosomal Subunit Assembly Defects ofFrontiers in microbiology · 2019Article
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- Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Frontiers in physiology · 2018Article
- Transcriptome and Proteome of Fish-PathogenicFrontiers in microbiology · 2018Article
- Xerotolerant bacteria: surviving through a dry spell.Nature reviews. Microbiology · 2017Review
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An integrated transcriptomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass chilling in cold air. The response of E. coli during exponential growth at 25 °C a(w) 0.985, 14 °C a(w) 0.985, 25 °C a(w) 0.967, and 14 °C a(w) 0.967 was compared with that of a reference culture (35 °C a(w) 0.993). Gene and protein expression profiles of E. coli were more strongly affected by low water activity (a(w) 0.967) than by low temperature (14 °C). Predefined group enrichment analysis revealed that a universal response of E. coli to all test conditions included activation of the master stress response regulator RpoS and the Rcs phosphorelay system involved in the biosynthesis of the exopolysaccharide colanic acid, as well as down-regulation of elements involved in chemotaxis and motility. However, colanic acid-deficient mutants were shown to achieve comparable growth rates to their wild-type parents under all conditions, indicating that colanic acid is not required for growth. In contrast to the transcriptomic data, the proteomic data revealed that several processes involved in protein synthesis were down-regulated in overall expression at 14 °C a(w) 0.985, 25 °C a(w) 0.967, and 14 °C a(w) 0.967. This result suggests that during growth under these conditions, E. coli, although able to transcribe the required mRNA, may lack the cellular resources required for translation. Elucidating the global adaptive response of E. coli O157:H7 during exposure to chilling and water activity stress has provided a baseline of knowledge of the physiology of this pathogen.
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