ArticleBiomicrofluidics2011
Isolation and manipulation of living adherent cells by micromolded magnetic rafts.
Article in Biomicrofluidics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Chip Calorimetry for Single-Cell Analysis: Advances, Challenges, and Opportunities.Sensors (Basel, Switzerland) · 2026Review
- Integrating single-cell transcriptomics with cellular phenotypes: cell morphology, CaBiophysical reviews · 2024Review
- Microraft arrays for serial-killer CD19 chimeric antigen receptor T cells and single cell isolation.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- Scalable Additive Construction of Arrayed Microstructures with Encoded Properties for Bioimaging.Micromachines · 2022Article
- See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity.Communications biology · 2022Article
- A technology of a different sort: microraft arrays.Lab on a chip · 2021Review
- Bio-assembling and Bioprinting for Engineering Microvessels from the Bottom Up.International journal of bioprinting · 2021Review
- Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform.IEEE transactions on bio-medical engineering · 2020Article
- Automated platform for cell selection and separation based on four-dimensional motility and matrix degradation.The Analyst · 2020Article
- Microraft array-based platform for sorting of viable microcolonies based on cell-lethal immunoassay of intracellular proteins in microcolony biopsies.The Analyst · 2020Article
- Automated sensing and splitting of stem cell colonies on microraft arrays.APL bioengineering · 2019Article
- Characterization of Tensioned PDMS Membranes for Imaging Cytometry on Microraft Arrays.Analytical chemistry · 2018Article
- A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology.Cellular and molecular gastroenterology and hepatology · 2018Article
- Highly efficient cellular cloning using Ferro-core Micropallet Arrays.Scientific reports · 2017Article
- Single cell biology beyond the era of antibodies: relevance, challenges, and promises in biomedical research.Cellular and molecular life sciences : CMLS · 2017Review
- Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Selective single cell isolation for genomics using microraft arrays.Nucleic acids research · 2016Article
- Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots.Nature communications · 2016Article
- Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism.Journal of cellular and molecular medicine · 2016Article
- Array-Based Platform To Select, Release, and Capture Epstein-Barr Virus-Infected Cells Based on Intercellular Adhesion.Analytical chemistry · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A new strategy for magnetically manipulating and isolating adherent cells with extremely high post-collection purity and viability is reported. Micromolded magnetic elements (termed microrafts) were fabricated in an array format and used as culture surfaces and carriers for living, adherent cells. A poly(styrene-co-acrylic acid) polymer containing well dispersed magnetic nanoparticles was developed for creating the microstructures by molding. Nanoparticles of γFe(2)O(3) at concentrations up to 1% wt.∕wt. could be used to fabricate microrafts that were optically transparent, highly magnetic, biocompatible, and minimally fluorescent. To prevent cellular uptake of nanoparticles from the magnetic polymer, a poly(styrene-co-acrylic acid) layer lacking γFe(2)O(3) nanoparticles was placed over the initial magnetic microraft layer to prevent cellular uptake of the γFe(2)O(3) during culture. The microraft surface geometry and physical properties were altered by varying the polymer concentration or layering different polymers during fabrication. Cells plated on the magnetic microrafts were visualized using standard imaging techniques including brightfield, epifluorescence, and confocal microscopy. Magnetic microrafts possessing cells of interest were dislodged from the array and efficiently collected with an external magnet. To demonstrate the feasibility of cell isolation using the magnetic microrafts, a mixed population of wild-type cells and cells stably transfected with a fluorescent protein was plated onto an array. Microrafts possessing single, fluorescent cells were released from the array and magnetically collected. A post-sorting single-cell cloning rate of 92% and a purity of 100% were attained.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.