ArticleProceedings of the National Academy of Sciences of the United States of America2011
Tobacco nicotine uptake permease (NUP1) affects alkaloid metabolism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 122 citations in OpenAlex.
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- Advances in Functional Genomics and Biotechnology for Enhancing Therapeutic Potential of Medicinal Plants.International journal of molecular sciences · 2026Review
- Integrated transcriptomic and metabolomic analyses reveal the regulatory network underlying NtGSTU10-mediated nicotine synthesis and transport in tobacco.Scientific reports · 2026Article
- Cell-type-specific alkaloid and terpenoid biosynthesis in glandular trichomes: single-cell and spatial transcriptomic perspectives.Frontiers in plant science · 2026Review
- Transport of secondary metabolites in plants: Mechanistic insights and transporter engineering for crop improvement.Plant communications · 2025Review
- Genomic structure of yellow lupin (Lupinus luteus): genome organization, evolution, gene family expansion, metabolites and protein synthesis.BMC genomics · 2025Article
- Integration of co-culture and transport engineering for enhanced metabolite production.Plant biotechnology (Tokyo, Japan) · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Multiomics comparison among populations of three plant sources of Amomi Fructus.Horticulture research · 2023Article
- Terpenoid Transport in Plants: How Far from the Final Picture?Plants (Basel, Switzerland) · 2023Review
- Functional identification of purine permeases reveals their roles in caffeine transport in tea plants (Frontiers in plant science · 2022Article
- Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Frontiers in plant science · 2022Article
- Alkaloid Biosynthesis in the Early Stages of the Germination ofPlants (Basel, Switzerland) · 2021Article
- Engineering cellular metabolite transport for biosynthesis of computationally predicted tropane alkaloid derivatives in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Identification and functional characterization of a PDR transporter in Tripterygium wilfordii Hook.f. that mediates the efflux of triptolide.Plant molecular biology · 2021Article
- Effects of Oral pH Changes on Smoking Desire.Balkan medical journal · 2021Article
- Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?International journal of molecular sciences · 2021Review
- Harnessing a Transient Gene Expression System inPlants (Basel, Switzerland) · 2021Article
- Membrane transporters: the key drivers of transport of secondary metabolites in plants.Plant cell reports · 2021Review
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An effective plant alkaloid chemical defense requires a variety of transport processes, but few alkaloid transporters have been characterized at the molecular level. Previously, a gene fragment encoding a putative plasma membrane proton symporter was isolated, because it was coordinately regulated with several nicotine biosynthetic genes. Here, we show that this gene fragment corresponds to a Nicotiana tabacum gene encoding a nicotine uptake permease (NUP1). NUP1 belongs to a plant-specific class of purine uptake permease-like transporters that originated after the bryophytes but before or within the lycophytes. NUP1 expressed in yeast cells preferentially transported nicotine relative to other pyridine alkaloids, tropane alkaloids, kinetin, and adenine. NUP1-GFP primarily localized to the plasma membrane of tobacco Bright Yellow-2 protoplasts. WT NUP1 transcripts accumulated to high levels in the roots, particularly in root tips. NUP1-RNAi hairy roots had reduced NUP1 mRNA accumulation levels, reduced total nicotine levels, and increased nicotine accumulation in the hairy root culture media. Regenerated NUP1-RNAi plants showed reduced foliar and root nicotine levels as well as increased seedling root elongation rates. Thus, NUP1 affected nicotine metabolism, localization, and root growth.
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