ArticlePlant cell reports2012
Identification of suitable reference genes for real-time RT-PCR normalization in the grapevine-downy mildew pathosystem.
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries.Plant methods · 2021Article
- The grapevine (Vitis vinifera L.) floral transcriptome in Pinot noir variety: identification of tissue-related gene networks and whorl-specific markers in pre- and post-anthesis phases.Horticulture research · 2021Article
- Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica).Scientific reports · 2021Article
- Review
- Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.Scientific reports · 2020Article
- Differential expression of transcription factor- and further growth-related genes correlates with contrasting cluster architecture in Vitis vinifera 'Pinot Noir' and Vitis spp. genotypes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Integrating metabolomics and targeted gene expression to uncover potential biomarkers of fungal/oomycetes-associated disease susceptibility in grapevine.Scientific reports · 2020Article
- Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses.FEBS open bio · 2020Article
- Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications.Frontiers in plant science · 2020Article
- Transcriptional regulation of stilbene synthases in grapevine germplasm differentially susceptible to downy mildew.BMC plant biology · 2019Article
- Elevated atmospheric COScientific reports · 2019Article
- Selection of reliable reference genes for RT-qPCR during methyl jasmonate, salicylic acid and hydrogen peroxide treatments in Ganoderma lucidum.World journal of microbiology & biotechnology · 2018Article
- Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola.International journal of molecular sciences · 2018Article
- Molecular cloning of a CC-NBS-LRR gene from Vitis quinquangularis and its expression pattern in response to downy mildew pathogen infection.Molecular genetics and genomics : MGG · 2018Article
- Comparative analysis of constitutive proteome between resistant and susceptible tomato genotypes regarding to late blight.Functional & integrative genomics · 2018Article
- Identification of appropriate reference genes for RT-qPCR analysis in Juglans regia L.PloS one · 2018Article
- Identification of suitable grapevine reference genes for qRT-PCR derived from heterologous species.Molecular genetics and genomics : MGG · 2016Article
- Reference gene selection for normalization of RT-qPCR gene expression data from Actinidia deliciosa leaves infected with Pseudomonas syringae pv. actinidiae.Scientific reports · 2015Article
- Genome-Wide Identification and Validation of Reference Genes in Infected Tomato Leaves for Quantitative RT-PCR Analyses.PloS one · 2015Article
- Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR).BMC research notes · 2014Article
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Authors and funding
6 authors.
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Abstract
Due to its reproducibility and sensitivity, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) has become the method of choice for quantifying gene expression. However, the accuracy of RT-qPCR is prone to bias if proper precautions are not taken, e.g. starting with intact, non-degraded RNA, considering the PCR efficiency and using the right reference gene(s) for normalization. It has been reported that some of the well-known reference genes are differentially regulated under certain experimental conditions suggesting that there is no gene that could be used as a universal reference. This paper aims at selecting the most suitable reference gene(s) out of six putative genes to be used as normalizer(s) for quantification of gene expression in the grapevine-downy mildew interaction as well as upon induced resistance with chemical elicitors. Moreover, the paper aims at determining the optimal number of reference genes to be used in normalization, since it has been emphasized in the literature that using multiple reference genes increases accuracy. Two different software tools, geNorm and Normfinder, were used to identify the most stable reference genes in grapevine under the aforementioned conditions. The importance of the choice of adequate reference genes is highlighted by studying chitinase expression.
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