ArticleMolecular and cellular biology2011
Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.
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Who cites it
121 citing papers in PubMed, 248 citations in OpenAlex.
- Membranes arrest the coarsening of mitochondrial condensates in human cells.Communications biology · 2026Article
- Mitochondrial pearling: membrane biophysics, CaFrontiers in cell and developmental biology · 2026Review
- Mitochondrial curation for the next generation.Current opinion in genetics & development · 2025Review
- Mitochondrial DNA copy number alterations: Key players in the complexity of glioblastoma (Review).Molecular medicine reports · 2025Review
- Revealing mitochondrial architecture and functions with single molecule localization microscopy.Biology of the cell · 2025Review
- OPA1 and disease-causing mutants perturb mitochondrial nucleoid distribution.Cell death & disease · 2024Article
- Physico-Chemical Properties of CdTe/Glutathione Quantum Dots Obtained by Microwave Irradiation for Use in Monoclonal Antibody and Biomarker Testing.Nanomaterials (Basel, Switzerland) · 2024Article
- Uncovering the impact of UV radiation on mitochondria in dermal cells: a STED nanoscopy study.Scientific reports · 2024Article
- Review
- Revisiting the Mitochondrial Function and Communication in Neurodegenerative Diseases.Current pharmaceutical design · 2024Review
- mtFociCounter for automated single-cell mitochondrial nucleoid quantification and reproducible foci analysis.Nucleic acids research · 2023Article
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- 35 Years of TFAM Research: Old Protein, New Puzzles.Biology · 2023Review
- The C-Terminal Tail of Mitochondrial Transcription Factor A Is Dispensable for Mitochondrial DNA Replication and Transcription In Situ.International journal of molecular sciences · 2023Article
- Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.Scientific reports · 2023Article
- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence.Light, science & applications · 2023Article
- Measurement of Nucleoid Size Using STED Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation.Nature communications · 2022Article
- Article
- DarT-mediated mtDNA damage induces dynamic reorganization and selective segregation of mitochondria.The Journal of cell biology · 2022Article
61 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
A fundamental objective in molecular biology is to understand how DNA is organized in concert with various proteins, RNA, and biological membranes. Mitochondria maintain and express their own DNA (mtDNA), which is arranged within structures called nucleoids. Their functions, dimensions, composition, and precise locations relative to other mitochondrial structures are poorly defined. Superresolution fluorescence microscopy techniques that exceed the previous limits of imaging within the small and highly compartmentalized mitochondria have been recently developed. We have improved and employed both two- and three-dimensional applications of photoactivated localization microscopy (PALM and iPALM, respectively) to visualize the core dimensions and relative locations of mitochondrial nucleoids at an unprecedented resolution. PALM reveals that nucleoids differ greatly in size and shape. Three-dimensional volumetric analysis indicates that, on average, the mtDNA within ellipsoidal nucleoids is extraordinarily condensed. Two-color PALM shows that the freely diffusible mitochondrial matrix protein is largely excluded from the nucleoid. In contrast, nucleoids are closely associated with the inner membrane and often appear to be wrapped around cristae or crista-like inner membrane invaginations. Determinations revealing high packing density, separation from the matrix, and tight association with the inner membrane underscore the role of mechanisms that regulate access to mtDNA and that remain largely unknown.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.