ArticleInsect biochemistry and molecular biology2011
Manduca sexta moricin promoter elements can increase promoter activities of Drosophila melanogaster antimicrobial peptide genes.
Article in Insect biochemistry and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Review
- A Toll-Spätzle Pathway in the Immune Response ofInsects · 2020Article
- Molecular Cloning and Characterization of Five Glutathione S-Transferase Genes and Promoters fromInsects · 2020Article
- Molecular Identification of a Moricin Family Antimicrobial Peptide (Px-Mor) FromFrontiers in microbiology · 2019Article
- Transcription Factor Forkhead Regulates Expression of Antimicrobial Peptides in the Tobacco Hornworm, Manduca sexta.Scientific reports · 2017Article
- Co-expression of Dorsal and Rel2 Negatively Regulates Antimicrobial Peptide Expression in the Tobacco Hornworm Manduca sexta.Scientific reports · 2016Article
- The immune signaling pathways of Manduca sexta.Insect biochemistry and molecular biology · 2015Article
- Insect antimicrobial peptides and their applications.Applied microbiology and biotechnology · 2014Review
- Identification of conserved and novel microRNAs in Manduca sexta and their possible roles in the expression regulation of immunity-related genes.Insect biochemistry and molecular biology · 2014Article
- Article
- Manduca sexta gloverin binds microbial components and is active against bacteria and fungi.Developmental and comparative immunology · 2012Article
- A Toll-Spätzle pathway in the tobacco hornworm, Manduca sexta.Insect biochemistry and molecular biology · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Insects produce a variety of antimicrobial peptides (AMPs). Induction of insect AMP genes is regulated by the Toll and IMD (immune deficiency) pathways via NF-κB and GATA factors. Little is known about species-specific regulation of AMP genes. In this report, we showed that activities of most Manduca sexta and Drosophila melanogaster AMP gene promoters were regulated in a species-specific manner in Drosophila (Dipteran) S2 cells and Spodoptera frugiperda (Lepidopteran) Sf9 cells. A κB-GATA element (22 bp) from M. sexta moricin (MsMoricin) promoter could significantly increase activities of Drosophila AMP gene promoters in S2 cells, and an MsMoricin promoter activating element (MPAE) (140 bp) could increase activity of drosomycin promoter specifically in Sf9 cells. However, κB and GATA factors alone were not sufficient for MsMoricin gene activation, suggesting that other co-regulators may be required to fully activate AMP genes. Our results suggest that induction of insect AMP genes may require a transcription complex composed of common nuclear factors (such as NF-κB and GATA factors) and species-related co-regulators, and it is the co-regulators that may confer species-specific regulation of AMP genes. In addition, we showed that activity of Drosophila drosomycin promoter could be activated cooperatively by the inserted exogenous κB-GATA element and the endogenous κB element. These findings revealed an approach of engineering AMP genes with enhanced activities, which may lead to broad applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.