ArticleBiophysical journal2011
Vesicle fusion observed by content transfer across a tethered lipid bilayer.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- Programmable RNA Loading of Extracellular Vesicles with Toehold-Release Purification.Journal of the American Chemical Society · 2024Article
- Membrane localization accelerates association under conditions relevant to cellular signaling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A coarse-grained simulation model for colloidal self-assemblySoft matter · 2023Article
- Measuring single-virus fusion kinetics using an assay for nucleic acid exposure.Biophysical journal · 2022Article
- Review
- Single-virus content-mixing assay reveals cholesterol-enhanced influenza membrane fusion efficiency.Biophysical journal · 2021Article
- A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics.Journal of visualized experiments : JoVE · 2020Article
- Article
- Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.Biophysical journal · 2019Article
- Phase-Separated Liposomes Enhance the Efficiency of Macromolecular Delivery to the Cellular Cytoplasm.Cellular and molecular bioengineering · 2017Article
- Vesicle Fusion Mediated by Solanesol-Anchored DNA.Biophysical journal · 2017Article
- SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes.Biophysical journal · 2017Article
- Artificial Membrane Fusion Triggered by Strain-Promoted Alkyne-Azide Cycloaddition.Bioconjugate chemistry · 2017Article
- SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy.Journal of visualized experiments : JoVE · 2016Article
- DNA Nanostructures on Membranes as Tools for Synthetic Biology.Biophysical journal · 2016Review
- High cholesterol obviates a prolonged hemifusion intermediate in fast SNARE-mediated membrane fusion.Biophysical journal · 2015Article
- Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins.Critical reviews in biochemistry and molecular biology · 2015Review
- DNA-based patterning of tethered membrane patches.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Individual vesicle fusion events mediated by lipid-anchored DNA.Biophysical journal · 2013Article
- Constructing droplet interface bilayers from the contact of aqueous droplets in oil.Nature protocols · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target architecture that allows observation of content transfer of individual vesicles across the tethered planar bilayer. Tethering and fusion are mediated by hybridization of complementary DNA-lipid conjugates inserted into the two membranes, and content transfer is monitored by the dequenching of an aqueous content dye. By analyzing the diffusion profile of the aqueous dye after vesicle fusion, we are able to distinguish content transfer across the tethered bilayer patch from vesicle leakage above the patch.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.