ArticleBiophysical journal2011
Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Effects of Membrane Cholesterol on the Structure and Function of Selected Class A GPCRs─Challenges and Future Perspectives.Biochemistry · 2025Review
- Cholesterol Dependent Activity of the Adenosine AMolecules (Basel, Switzerland) · 2022Article
- Allosteric modulation of the adenosine AeLife · 2022Article
- In Situ Reconstitution of the Adenosine AJournal of the American Chemical Society · 2017Article
- ASLAS discovery : advancing life sciences R & D · 2017Article
- Article
- Impact of purification conditions and history on A2A adenosine receptor activity: The role of CHAPS and lipids.Protein expression and purification · 2016Article
- Membrane proteins, detergents and crystals: what is the state of the art?Acta crystallographica. Section F, Structural biology communications · 2014Review
- How well does cholesteryl hemisuccinate mimic cholesterol in saturated phospholipid bilayers?Journal of molecular modeling · 2014Article
- Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2.Biochimica et biophysica acta · 2013Article
- Ligand-dependent cholesterol interactions with the human A(2A) adenosine receptor.Chemistry and physics of lipids · 2013Article
- β2-Adrenergic receptor solutions for structural biology analyzed with microscale NMR diffusion measurements.Angewandte Chemie (International ed. in English) · 2013Article
- Methods for recombinant expression and functional characterization of human cannabinoid receptor CB2.Computational and structural biotechnology journal · 2013Review
- Predictions for cholesterol interaction sites on the A2A adenosine receptor.Journal of the American Chemical Society · 2012Article
- Agonist dynamics and conformational selection during microsecond simulations of the A(2A) adenosine receptor.Biophysical journal · 2012Article
- Characterisation of Conformational and Ligand Binding Properties of Membrane Proteins Using Synchrotron Radiation Circular Dichroism (SRCD).Advances in experimental medicine and biologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Although reconstitution of membrane proteins within protein detergent complexes is often used to enable their structural or biophysical characterization, it is unclear how one should rationally choose the appropriate micellar environment to preserve native protein folding. Here, we investigated model mixed micelles consisting of a nonionic glucosylated alkane surfactant from the maltoside and thiomaltoside families, bile salt surfactant, and the steryl derivative cholesteryl hemisuccinate. We correlated several key attributes of these micelles with the in vitro ligand-binding activity of hA(2)aR in these systems. Through small-angle neutron scattering and radioligand-binding analysis, we found several key aspects of mixed micellar systems that preserve the activity of hA(2)aR, including a critical amount of cholesteryl hemisuccinate per micelle, and an optimal hydrophobic thickness of the micelle that is analogous to the thickness of native mammalian bilayers. These features are closely linked to the headgroup chemistry of the surfactant and the hydrocarbon chain length, which influence both the morphology and composition of resulting micelles. This study should serve as a general guide for selecting the appropriate mixed surfactant systems to stabilize membrane proteins for biophysical analysis.
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