ArticleBiophysical journal2011
Lipid bilayer mechanics in a pipette with glass-bilayer adhesion.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Reducing agents facilitate membrane patch seal integrity and longevity.Channels (Austin, Tex.) · 2024Article
- Programmable Lipid Bilayer Tension-Control Apparatus for Quantitative Mechanobiology.ACS nano · 2024Article
- Activation of Piezo1 but Not NaUltrasound in medicine & biology · 2018Article
- Mechano-sensitization of mammalian neuronal networks through expression of the bacterial large-conductance mechanosensitive ion channel.Journal of cell science · 2018Article
- Mechanical Transduction and the Dark Energy of Biology.Biophysical journal · 2018Review
- Whole-GUV patch-clamping.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Influence of Global and Local Membrane Curvature on Mechanosensitive Ion Channels: A Finite Element Approach.Membranes · 2016Article
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Utrophin suppresses low frequency oscillations and coupled gating of mechanosensitive ion channels in dystrophic skeletal muscle.Channels (Austin, Tex.) · 2015Article
- Plasma membrane mechanical stress activates TRPC5 channels.PloS one · 2015Article
- Ion Channels in Native Chloroplast Membranes: Challenges and Potential for Direct Patch-Clamp Studies.Frontiers in physiology · 2015Review
- Gigaseal mechanics: creep of the gigaseal under the action of pressure, adhesion, and voltage.The journal of physical chemistry. B · 2014Article
- Biophysical implications of lipid bilayer rheometry for mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The purified mechanosensitive channel TREK-1 is directly sensitive to membrane tension.The Journal of biological chemistry · 2013Article
- Human PIEZO1: removing inactivation.Biophysical journal · 2013Article
- Article
- Molecular force transduction by ion channels: diversity and unifying principles.Journal of cell science · 2012Review
- Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Membrane permeable local anesthetics modulate Na(V)1.5 mechanosensitivity.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Electrophysiology is a central tool for measuring how different driving forces (e.g., ligand concentration, transmembrane voltage, or lateral tension) cause a channel protein to gate. Upon formation of the high resistance seal between a lipid bilayer and a glass pipette, the so-called "giga-seal", channel activity can be recorded electrically. In this article, we explore the implications of giga-seal formation on the mechanical state of a lipid bilayer patch. We use a mechanical model for the free energy of bilayer geometry in the presence of glass-bilayer adhesion to draw three potentially important conclusions. First, we use our adhesion model to derive an explicit relationship between applied pressure and patch shape that is consistent with the Laplace-Young Law, giving an alternative method of calculating patch tension under pressure. With knowledge of the adhesion constant, which we find to be in the range ∼0.4-4 mN/m, and the pipette size, one can precisely calculate the patch tension as a function of pressure, without the difficultly of obtaining an optical measurement of the bilayer radius of curvature. Second, we use data from previous electrophysiological experiments to show that over a wide range of lipids, the resting tension on a electrophysiological patch is highly variable and can be 10-100 times higher than estimates of the tension in a typical cell membrane. This suggests that electrophysiological experiments may be systematically altering channel-gating characteristics and querying the channels under conditions that are not the same as their physiological counterparts. Third, we show that reversible adhesion leads to a predictable change in the population response of gating channels in a bilayer patch.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.