ArticleBMC genomics2011
Whole genome analysis of linezolid resistance in Streptococcus pneumoniae reveals resistance and compensatory mutations.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 79 citations in OpenAlex.
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- Antimicrobial peptides: structure, functions and translational applications.Nature reviews. Microbiology · 2025Review
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- ESKAPE pathogens: antimicrobial resistance, epidemiology, clinical impact and therapeutics.Nature reviews. Microbiology · 2024Review
- New Resistance Mutations Linked to Decreased Susceptibility to Solithromycin in Streptococcus pneumoniae Revealed by Chemogenomic Screens.Antimicrobial agents and chemotherapy · 2023Article
- Role of PatAB Transporter in Efflux of Levofloxacin inAntibiotics (Basel, Switzerland) · 2022Article
- Whole-Genome Transformation of Yeast Promotes Rare Host Mutations with a Single Causative SNP Enhancing Acetic Acid Tolerance.Molecular and cellular biology · 2022Article
- Mechanisms of antibiotic action shape the fitness landscapes of resistance mutations.Computational and structural biotechnology journal · 2022Article
- Multidrug-Resistant Bacteria: Their Mechanism of Action and Prophylaxis.BioMed research international · 2022Review
- A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors.PLoS genetics · 2021Article
- Article
- In vitro activity of radezolid against Enterococcus faecium and compared with linezolid.The Journal of antibiotics · 2020Article
- Resistance in Vancomycin-Resistant Enterococci.Infectious disease clinics of North America · 2020Review
- Gain- and Loss-of-Function Screens Coupled to Next-Generation Sequencing for Antibiotic Mode of Action and Resistance Studies inAntimicrobial agents and chemotherapy · 2019Article
- Molecular Analysis of Linezolid-Resistant Clinical Isolates ofAntimicrobial agents and chemotherapy · 2019Article
- Alterations in Life-History Associated With Non-target-site Herbicide Resistance inFrontiers in plant science · 2019Article
- Assess drug-resistance phenotypes, not just genotypes.Nature microbiology · 2016Article
- The genomic basis of adaptation to the fitness cost of rifampicin resistance in Pseudomonas aeruginosa.Proceedings. Biological sciences · 2016Article
- A genomic approach to understand interactions between Streptococcus pneumoniae and its bacteriophages.BMC genomics · 2015Article
- Differences in antibiotic-induced oxidative stress responses between laboratory and clinical isolates of Streptococcus pneumoniae.Antimicrobial agents and chemotherapy · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundSeveral mutations were present in the genome of Streptococcus pneumoniae linezolid-resistant strains but the role of several of these mutations had not been experimentally tested. To analyze the role of these mutations, we reconstituted resistance by serial whole genome transformation of a novel resistant isolate into two strains with sensitive background. We sequenced the parent mutant and two independent transformants exhibiting similar minimum inhibitory concentration to linezolid.
resultsComparative genomic analyses revealed that transformants acquired G2576T transversions in every gene copy of 23S rRNA and that the number of altered copies correlated with the level of linezolid resistance and cross-resistance to florfenicol and chloramphenicol. One of the transformants also acquired a mutation present in the parent mutant leading to the overexpression of an ABC transporter (spr1021). The acquisition of these mutations conferred a fitness cost however, which was further enhanced by the acquisition of a mutation in a RNA methyltransferase implicated in resistance. Interestingly, the fitness of the transformants could be restored in part by the acquisition of altered copies of the L3 and L16 ribosomal proteins and by mutations leading to the overexpression of the spr1887 ABC transporter that were present in the original linezolid-resistant mutant.
conclusionsOur results demonstrate the usefulness of whole genome approaches at detecting major determinants of resistance as well as compensatory mutations that alleviate the fitness cost associated with resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.