ArticleAnalytical chemistry2011
Liquid chromatography-high resolution mass spectrometry analysis of fatty acid metabolism.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Characterizing ex vivo models for studying lipid metabolism in triple negative breast cancer.Journal of lipid research · 2026Article
- Metabolic Profile ofMetabolites · 2024Article
- Gut dysbiosis impairs intestinal renewal and lipid absorption in Scarb2 deficiency-associated neurodegeneration.Protein & cell · 2024Article
- Bio-Based Phase Change Materials for Sustainable Development.Materials (Basel, Switzerland) · 2024Article
- A novel CPT1A covalent inhibitor modulates fatty acid oxidation and CPT1A-VDAC1 axis with therapeutic potential for colorectal cancer.Redox biology · 2023Article
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- Potential of atmospheric pressure ionization sources for the analysis of free fatty acids in clinical and biological samples by gas chromatography-mass spectrometry.Analytical and bioanalytical chemistry · 2022Article
- Metabolomics as a potential tool for the diagnosis of growth hormone deficiency (GHD): a review.Archives of endocrinology and metabolism · 2021Review
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- Rumen fluid metabolomics of beef steers differing in feed efficiency.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Elevated Choline Kinase α-Mediated Choline Metabolism Supports the Prolonged Survival of TRAF3-Deficient B Lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Rumen Bacteria and Serum Metabolites Predictive of Feed Efficiency Phenotypes in Beef Cattle.Scientific reports · 2019Article
- Glutamine/glutamate metabolism rewiring in reprogrammed human hepatocyte-like cells.Scientific reports · 2019Article
- Human Cytomegalovirus pUL37x1 Is Important for Remodeling of Host Lipid Metabolism.Journal of virology · 2019Article
- Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry.Biomolecules · 2018Article
- Analytical Considerations of Stable Isotope Labelling in Lipidomics.Biomolecules · 2018Review
- IRE1α RNase-dependent lipid homeostasis promotes survival in Myc-transformed cancers.The Journal of clinical investigation · 2018Article
- Systems-level analysis of mechanisms regulating yeast metabolic flux.Science (New York, N.Y.) · 2016Article
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Authors and funding
5 authors.
Funding
Abstract
We present a liquid chromatography/mass spectrometry (LC/MS) method for long-chain and very-long-chain fatty acid analysis and its application to (13)C-tracer studies of fatty acid metabolism. Fatty acids containing 14 to 36 carbon atoms are separated by C(8) reversed-phase chromatography using a water-methanol gradient with tributylamine as ion pairing agent, ionized by electrospray and analyzed by a stand-alone orbitrap mass spectrometer. The median limit of detection is 5 ng/mL with a linear dynamic range of 100-fold. Ratios of unlabeled to (13)C-labeled species are quantitated precisely and accurately (average relative standard deviation 3.2% and deviation from expectation 2.3%). In samples consisting of fatty acids saponified from cultured mammalian cells, 45 species are quantified, with average intraday relative standard deviations for independent biological replicates of 11%. The method enables quantitation of molecular ion peaks for all labeled forms of each fatty acid. Different degrees of (13)C-labeling from glucose and glutamine correspond to fatty acid uptake from media, de novo synthesis, and elongation. To exemplify the utility of the method, we examined isogenic cell lines with and without activated Ras oncogene expression. Ras increases the abundance and alters the labeling patterns of saturated and monounsaturated very-long-chain fatty acids, with the observed pattern consistent with Ras leading to enhanced activity of ELOVL4 or an enzyme with similar catalytic activity. This LC/MS method and associated isotope tracer techniques should be broadly applicable to investigating fatty acid metabolism.
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