ArticleBiochemistry2011
Manipulating cofactor binding thermodynamics in an artificial oxygen transport protein.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Controlling heme redox properties in peptide amphiphile fibers with sequence and heme loading ratio.Biophysical journal · 2024Article
- Protein dynamics govern the oxyferrous state lifetime of an artificial oxygen transport protein.Biophysical journal · 2023Article
- Tailorable Tetrahelical Bundles as a Toolkit for Redox Studies.The journal of physical chemistry. B · 2022Article
- Liquid to solid transition of elastin condensates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Non-Covalent Coatings on Carbon Nanotubes Mediate Photosensitizer Interactions.ACS applied materials & interfaces · 2021Article
- Dynamics in natural and designed elastins and their relation to elastic fiber structure and recoil.Biophysical journal · 2021Article
- Designing heterotropically activated allosteric conformational switches using supercharging.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural Insight into the Mechanism of 4-Aminoquinolines Selectivity for the alpha2A-Adrenoceptor.Drug design, development and therapy · 2020Article
- Order, Disorder, and Temperature-Driven Compaction in a Designed Elastin Protein.The journal of physical chemistry. B · 2018Article
- Fast, cheap and out of control--Insights into thermodynamic and informatic constraints on natural protein sequences from de novo protein design.Biochimica et biophysica acta · 2016Article
- Preface.Methods in enzymology · 2016Article
- Protein design: toward functional metalloenzymes.Chemical reviews · 2014Review
- Engineering the assembly of heme cofactors in man-made proteins.Journal of the American Chemical Society · 2014Article
- Rational design of a zinc phthalocyanine binding protein.Journal of structural biology · 2014Article
- A three-dimensional printed cell for rapid, low-volume spectroelectrochemistry.Analytical biochemistry · 2013Article
- Dynamic factors affecting gaseous ligand binding in an artificial oxygen transport protein.Biochemistry · 2013Article
- Modulation of function in a minimalist heme-binding membrane protein.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2012Article
- Manipulating Reduction Potentials in an Artificial Safranin Cofactor.Tetrahedron letters · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We report the mutational analysis of an artificial oxygen transport protein, HP7, which operates via a mechanism akin to that of human neuroglobin and cytoglobin. This protein destabilizes one of two heme-ligating histidine residues by coupling histidine side chain ligation with the burial of three charged glutamate residues on the same helix. Replacement of these glutamate residues with alanine, which is uncharged, increases the affinity of the distal histidine ligand by a factor of 13. Paradoxically, it also decreases heme binding affinity by a factor of 5 in the reduced state and 60 in the oxidized state. Application of a three-state binding model, in which an initial pentacoordinate binding event is followed by a protein conformational change to hexacoordinate, provides insight into the mechanism of this seemingly counterintuitive result: the initial pentacoordinate encounter complex is significantly destabilized by the loss of the glutamate side chains, and the increased affinity for the distal histidine only partially compensates for that. These results point to the importance of considering each oxidation and conformational state in the design of functional artificial proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.