ArticleACS chemical biology2012
RNA-Pt adducts following cisplatin treatment of Saccharomyces cerevisiae.
Article in ACS chemical biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 97 citations in OpenAlex.
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- Impacts of amino acid-linked platinum(II) complexes on DNA structure.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2025Article
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- Quantifying Intracellular Platinum Accumulation Using Inductively Coupled Mass Spectrometry.Current protocols · 2024Article
- Platinum-based drugs and hydrogel: a promising anti-tumor combination.Drug delivery · 2023Review
- Inhibition of nucleolar transcription by oxaliplatin involves ATM/ATR kinase signaling.Cell chemical biology · 2023Article
- PD-1/PD-L1 and DNA Damage Response in Cancer.Cells · 2023Review
- NSAID-Based Coordination Compounds for Biomedical Applications: Recent Advances and Developments.International journal of molecular sciences · 2022Review
- Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.RNA (New York, N.Y.) · 2021Article
- Investigation on Optical and Biological Properties of 2-(4-Dimethylaminophenyl)benzothiazole Based Cycloplatinated Complexes.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Human ribosomal G-quadruplexes regulate heme bioavailability.The Journal of biological chemistry · 2020Article
- Nucleolar Stress Induction by Oxaliplatin and Derivatives.Journal of the American Chemical Society · 2019Article
- Cisplatin and beyond: molecular mechanisms of action and drug resistance development in cancer chemotherapy.Radiology and oncology · 2019Review
- Synergistic effect of meta-tetra(hydroxyphenyl)chlorin-based photodynamic therapy followed by cisplatin on malignant Hep-2 cells.OncoTargets and therapy · 2019Article
- Pt-induced crosslinks promote target enrichment and protection from serum nucleases.Journal of inorganic biochemistry · 2018Article
- Structural Elucidation of Cisplatin and HydratedACS omega · 2018Article
- Bacterial Cytological Profiling Reveals the Mechanism of Action of Anticancer Metal Complexes.Molecular pharmaceutics · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The numerous regulatory roles of cellular RNAs suggest novel potential drug targets, but establishing intracellular drug-RNA interactions is challenging. Cisplatin (cis-diamminedichloridoplatinum(II)) is a leading anticancer drug that forms exchange-inert complexes with nucleic acids, allowing its distribution on cellular RNAs to be followed ex vivo. Although Pt adduct formation on DNA is well-known, a complete characterization of cellular RNA-Pt adducts has not been performed. In this study, the action of cisplatin on S. cerevisiae in minimal media was established with growth curves, clonogenic assays, and tests for apoptotic markers. Despite high toxicity, cisplatin-induced apoptosis in S. cerevisiae was not observed under these conditions. In-cell Pt concentrations and Pt accumulation on poly(A)-mRNA, rRNA, total RNA, and DNA quantified via ICP-MS indicate ∼4- to 20-fold more Pt accumulation in total cellular RNA than in DNA. Interestingly, similar Pt accumulation is observed on rRNA and total RNA, corresponding to one Pt per (14,600 ± 1,500) and (5760 ± 580) nucleotides on total RNA following 100 and 200 μM cisplatin treatments, respectively. Specific Pt adducts mapped by primer extension analysis of a solvent-accessible 18S rRNA helix occur at terminal and internal loop regions and appear as soon as 1 h post-treatment. Pt per nucleotide accumulation on poly(A)-mRNA is 4- to 6-fold lower than on rRNA but could have consequences for low copy-number or highly regulated transcripts. Taken together, these data demonstrate significant accumulation of Pt adducts on cellular RNA species following in cellulo cisplatin treatment. These and other small molecule-RNA interactions could disrupt processes regulated by RNA.
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Registered trials
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