ArticlePloS one2011
An atypical epigenetic mechanism affects uniparental expression of Pol IV-dependent siRNAs.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Base Excision DNA Repair in Plants:International journal of molecular sciences · 2023Review
- Small RNA-mediated DNA methylation during plant reproduction.The Plant cell · 2023Review
- Abundant expression of maternal siRNAs is a conserved feature of seed development.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-wide discovery and validation of Eucalyptus small RNAs reveals variable patterns of conservation and diversity across species of Myrtaceae.BMC genomics · 2015Article
- Evolution and function of genomic imprinting in plants.Genes & development · 2015Review
- RNA-directed DNA methylation: an epigenetic pathway of increasing complexity.Nature reviews. Genetics · 2014Review
- Article
- RNA-directed DNA methylation regulates parental genomic imprinting at several loci in Arabidopsis.Development (Cambridge, England) · 2013Article
- Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond.Nature reviews. Genetics · 2013Review
- Article
- Epigenetic Modifications during Angiosperm Gametogenesis.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundSmall RNAs generated by RNA polymerase IV (Pol IV) are the most abundant class of small RNAs in flowering plants. In Arabidopsis thaliana Pol IV-dependent short interfering (p4-si)RNAs are imprinted and accumulate specifically from maternal chromosomes in the developing seeds. Imprinted expression of protein-coding genes is controlled by differential DNA or histone methylation placed in gametes. To identify epigenetic factors required for maternal-specific expression of p4-siRNAs we analyzed the effect of a series of candidate mutations, including those required for genomic imprinting of protein-coding genes, on uniparental expression of a representative p4-siRNA locus.
resultsPaternal alleles of imprinted genes are marked by DNA or histone methylation placed by DNA METHYLTRANSFERASE 1 or the Polycomb Repressive Complex 2. Here we demonstrate that repression of paternal p4-siRNA expression at locus 08002 is not controlled by either of these mechanisms. Similarly, loss of several chromatin modification enzymes, including a histone acetyltransferase, a histone methyltransferase, and two nucleosome remodeling proteins, does not affect maternal expression of locus 08002. Maternal alleles of imprinted genes are hypomethylated by DEMETER DNA glycosylase, yet expression of p4-siRNAs occurs irrespective of demethylation by DEMETER or related glycosylases.
conclusionsDifferential DNA methylation and other chromatin modifications associated with epigenetic silencing are not required for maternal-specific expression of p4-siRNAs at locus 08002. These data indicate that there is an as yet unknown epigenetic mechanism causing maternal-specific p4-siRNA expression that is distinct from the well-characterized mechanisms associated with DNA methylation or the Polycomb Repressive Complex 2.
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